期刊文献+

Chemical Synthesis and Cloning of Overlapping Concatenated Human Atrial Natriuretic Factor Gene with Gene Engineering

Chemical Synthesis and Cloning of Overlapping Concatenated Human Atrial Natriuretic Factor Gene with Gene Engineering
下载PDF
导出
摘要 Eight oligonucleotide fragments were designed with the aid of a computer and synthesizedaccording to the amino add sequcnce of human atrial natriuretic factor(ANF).By means of an-nealing and ligation,these fragments were assembled into an overlapping concatenator consisting oftwo ANF genes ligated by TGATG for termination and initiation of translation.Theconcatenator was omserted into plasmid pRC23 and the recobinant DNA was transformed into E.coli strain TAP106.Analysis by restriction enzyme mapping,hybridization and DNA sequenongshowed that the orientation and reading frame of the gene were correct. Eight oligonucleotide fragments were designed with the aid of a computer and synthesized according to the amino add sequcnce of human atrial natriuretic factor(ANF).By means of an- nealing and ligation,these fragments were assembled into an overlapping concatenator consisting of two ANF genes ligated by TGATG for termination and initiation of translation.The concatenator was omserted into plasmid pRC23 and the recobinant DNA was transformed into E. coli strain TAP106.Analysis by restriction enzyme mapping,hybridization and DNA sequenong showed that the orientation and reading frame of the gene were correct.
出处 《Journal of Medical Colleges of PLA(China)》 CAS 1989年第2期181-185,187,共6页 中国人民解放军军医大学学报(英文版)
关键词 atrial NATRIURETIC factor PLASMID DNA synthesis OVERLAPPING CONCATENATED GENE atrial natriuretic factor plasmid DNA synthesis overlapping concatenated gene
  • 相关文献

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部