摘要
目的:探讨奥曲肽(OCT)对胃癌细胞SGC-7901中Fas,FasL,P53,P21,P27和c-Myc表达的影响.方法:流式细胞术和RT-PCR法分别用于检测经OCT(1×10-7mol/L)处理前后胃癌细胞SGC-7901中Fas,FasL,P53,P21,P27,c-Myc阳性癌百分率、mRNA表达和蛋白表达及细胞周期G0/G1、S、G2/M的变化.结果:胃癌细胞SGC-7901经OCT诱导6、12、24和48h后,Fas和FasL mRNA表达均明显增加,P53mRNA表达则显著减少,而P21,P27和c-Myc mRNA表达均无明显变化;胃癌细胞SGC-7901经OCT诱导12h后,Fas和FasL蛋白相对表达强度也显著增加,P53蛋白相对表达强度则明显降低(5.5±0.3vs3.2±0.1,5.1±0.3vs4.5±0.1,3.3±0.2vs4.9±0.3,P<0.05或0.01),而P21,P27和c-Myc蛋白相对表达强度均无明显改变.细胞周期G0/G1、S、G2/M在OCT处理前后的变化并不明显.结论:OCT能上调SGC-7901胃癌细胞中Fas和FasL表达,下调突变型p53表达,是其诱导凋亡作用的重要机制之一.
AIM： To investigate whether OCT affects the expression of Fas, FasL, P53, P21, P27 and c-Myc in SGC-7901 HGC cells.METHODS： Expression percentage of Fas, FasL, P53, P21, P27, c-Myc-positive cells and G0/G1, S, G2/M stage were detected by using flow cy- tometry （FCM） with or without OCT treatment. Reverse transcription-polymerase chain reaction （RT-PCR） and FCM in SGC-7901 HGC cells were applied to evaluate the mRNA and protein expression levels of Fas, FasL, P53, P21, P27 and c-Myc in SGC-7901 HGC cells treated with and without OCT.RESULTS： After treatment with 1 x 10.7 mol/L OCT for 6, 12, 24, 48 h, although P53 mRNA expression was significantly decreased, Fas and FasL mRNA expression were both significantly increased in SGC-7901 HGC cells. However, P21, P27 and c-Myc mRNA expression did not significantly change in SGC-7901 HGC cells with or without OCT treatment. The relative intensity of Fas and FasL protein expression were also enhanced, but the relative intensity of P53 protein expression was decreased in SGC-7901 HGC cells （5.5 ± 0.3 vs 3.2 ± 0.1, 5.1 ± 0.3 vs 4.5 ± 0.1, 3.3 ± 0.2 vs 4.9 ± 0.3, P 〈 0.05 or 0.01）. The relative intensity of P21, P27 and c-myc protein expression did not change. However, G0/G1, S, G2/M stage did not significantly change in SGC-7901 HGC cells with or without OCT treatment.CONCLUSION： Our data suggest that OCT leading to apoptosis is associated with downregulation of mutant-type P53 and up-regulation of Fas and FasL in SGC-7901 HGC cells.
出处
《世界华人消化杂志》
CAS
北大核心
2009年第7期694-698,共5页
World Chinese Journal of Digestology
