摘要
以淀粉废水水解酸化处理系统中的蛋白酶、淀粉酶、脱氢酶为研究对象,考察了在无机离子、营养元素、pH等条件作用下的酶比活变化规律。实验结果表明:Mg2+和Co2+对酶比活有显著影响,MgCl2.6H2O加入量为30mg/L时蛋白酶、淀粉酶、脱氢酶的比活分别是空白的5.6倍、1.6倍、1.5倍,COD去除率提高17.6%;而CoCl2.6H2O加入量为10mg/L时,蛋白酶和淀粉酶的比活较单独投加30mg/L的MgCl2.6H2O时分别下降了80.6%和42.5%,脱氢酶比活却提高了13.2%;以NH4Cl与CO(NH2)2作为混合N源(m(NH4Cl)∶m(CO(NH2)2)=1∶2)时蛋白酶、淀粉酶、脱氢酶的比活分别是单独以CO(NH2)2为N源时的3.0倍、1.4倍、1.8倍,COD去除率提高了11.8%;中性偏碱性条件较利于酶促反应的进行。
Protease, amylase and dehydrogenase were used in the treatment of starch wastewater by the hydrolytic acidification system. The changes of the specific activities of these enzymes under different inorganic irons, nutrient elements and pH were studied. The experimental results show that： The effects of Mg^2＋ and Co^2＋ on specific enzyme activities are obvious; When the dosage of MgCl2 · 6H2O is 30 mg/L, the specific activities of protease, amylase and dehydrogenase are 5.6, 1.6, 1.5 times respectively higher than those in the blank tests and the COD removal rate is increased by 17.6% ; When the dosage of CoCl2· 6H2O is 10 mg/L, compared with the 30 mg/L MgCl2· 6H2O system, the specific activities of protease and amylase are decreased by 80. 6% and 42. 5% respectively, however, the specific activity of dehydrogenase is increased by 13.2% ; When NH4Cl and CO （NH2 ）2 with 1 ： 2 of mass ratio are used as nitrogen sources, the specific activities of protease, amylase and dehydrogenase are 3.0, 1.4, 1.8 times respectively higher than those using CO（ NH2 ）2 as nitrogen source, the COD removal rate is increased by 11.8% ; The partial neutral alkaline condition favors the enzymatic reaction.
出处
《化工环保》
CAS
CSCD
北大核心
2009年第2期118-121,共4页
Environmental Protection of Chemical Industry
关键词
水解酸化
淀粉废水
蛋白酶
淀粉酶
脱氢酶
酶比活
hydrolytic acidification
starch wastewater
protease
amylase
dehydrogenase
specific enzyme activity
