摘要
目的:探讨尿激酶对人血管内皮细胞基质金属蛋白酶9(Matrix metalloproteinase 9,MMP-9)表达的影响及机制。方法:将正常条件下培养的人脐静脉血管内皮细胞分为5组:第1组为空白对照,第2组加入尿激酶,第3、4、5组在加入尿激酶前分别加入MEK抑制剂PD98059、NF-κB抑制剂PDTC、AP-1抑制剂Curcumin。分别用RT-PCR和激光共聚焦技术检测细胞MMP-9 mRNA和蛋白表达量的变化。结果:第2组MMP-9 mRNA和蛋白表达量较第1组显著升高,其余3组较第2组显著降低。结论:尿激酶上调人血管内皮细胞MMP-9表达,其机制与Ras-Raf-MEK1/2-ERK1/2信号转导途径以及NF-κB和AP-1有关。
Objective： To investigate the effect of urokinase on the expression of Matrix metalloproteinase 9 （MMP-9） in human endothelial cell and its mechanism. Methods： The human umbilical vein endothelial cell cultured in normal condition were divided into five groups： the first group was blank control,the second group was given urokinase,the third, fourth and fifth groups were treated with MEK inhibitor PD98059,NF-κB inhibitor PDTC,and AP-1 inhibitor Curcumin,respectively before urokinase was added in. The expression of MMP-9 mRNA and protein in all the groups were detected by RT-PCR and Laser-Confocus respectively. Results： The mRNA and protein expression of MMP-9 in the second group were increased obviously, as compared with the first group. Compared with the second group,the mRNA and protein expression of MMP-9 in the third,fourth, and fifth groups were decreased significantly. Conclusion： Urokinase can npregulate the MMP-9 mRNA and protein expression of human endothelial cell. The mechanism is related to the Ras-Raf-MEK1/2-ERK1/2 signal transduction pathway, also to NF- κB and AP-1.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2009年第3期269-272,共4页
Journal of Chongqing Medical University
基金
重庆市科委课题(编号:CST200+BB5281)
