摘要
对毕赤酵母摇瓶发酵产木聚糖酶的培养条件进行优化。单因素实验结果表明,该菌产木聚糖酶的最佳碳源为麸皮,最佳氮源为酵母膏;通过正交实验得到工程菌的最佳摇瓶培养条件为30℃、接1种量10%、初始pH5.5、初始甲醇含量1.0%;甲醇最佳诱导时间为18h,经优化后,木聚糖酶酶活达到1765IU.ml-1。该酶耐热温度为70℃。
The flask liquid fermentation conditions for producing xylanase by Pichia pastoris were investigated. The prime flask cultivate condition was obtained by using orthogo- naldesign： temp 30℃, inoculate quantity 10%, pH 5.5, methanol 1.0% . The xylanase activity reached about 1765 IU. mL^-1 after the medium was optimized. The methanol induction optimized time point was 18 h .The optimum temperature of xylanase was 70℃.
出处
《江西化工》
2009年第1期69-71,共3页
Jiangxi Chemical Industry
关键词
毕赤酵母
木聚糖酶
正交试验
Pichia pastoris xylanase orthogonal experiment
