摘要
用壳聚糖包裹的硅胶为载体,利用壳聚糖表面的氨基与戊二醛结合,在硅胶表面引入醛基,固定模板蛋白(牛血红蛋白).用溶胶-凝胶过程再次包裹固定蛋白质的载体,洗去模板蛋白后,得到具有选择性识别的牛血红蛋白分子印迹聚合物.用红外光谱(IR)、扫描电子显微镜(SEM)和元素分析对聚合物进行了表征,结果表明,载体表面成功地接枝了分子印迹聚合物.选择性吸附实验结果表明,分子印迹聚合物具有良好的识别性能,能实现水溶液中牛血红蛋白的富集.
The silica modified with chitosan was synthesized as a carrier material. The glutaraldehyde was coupled with amido group of chitosan, and template protein-bovine hemoglobin was immobilized by aldehyde group. Then, the carrier was coated by sol-gel, after template protein was removed; molecularly imprinted polymer was prepared for selectively recognizing bovine hemoglobin （BHb）. The results of IR and scanning electron microscope（SEM） demonstrated the formation of a grafting polymer layer on the modified silica gel surface. Selectivity adsorption experiments under equilibrium adsorption show that the imprinted polymers have good adsorption capacity and specific recognition for BHb in aqueous solution, and imprinted polymer hold good recognition capability for BHb when completive substances are present. The MIP synthesis strategy is promising for separating and purifying specific protein compositions in bio-samples. It may have good prospect for riching target protein, which is required to further study.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2009年第4期691-696,共6页
Chemical Journal of Chinese Universities
基金
国家“九七三”计划(批准号:2007CB914100)
国家自然科学基金(批准号:20675040,20875049,20875050)资助
关键词
分子印迹聚合物
牛血红蛋白
硅胶
壳聚糖
Molecular imprinted polymer
Bovine hemoglobin
Silica gel
Chitosan
