摘要
为了探讨几种化合物对温石棉促人胚肺（ＨＥＬ）细胞增殖作用的影响，用不同浓度的柠檬酸铝、混合稀土或亚硒酸钠溶液浸泡温石棉１小时后，再将其与人胚肺细胞共同孵育，通过测定［３Ｈ］ＴｄＲ掺入量，来了解人胚肺细胞的增殖状况。结果显示，２．５～１０μｇ／ｍｌ的温石棉、时间作用在２４小时之内，或２０～８０μｇ／ｍｌ温石棉、时间作用在６小时之内均可促使人胚肺细胞增殖。与未处理温石棉组相比，经三种化合物处理的温石棉其促细胞增殖作用明显下降。提示，采用三种化合物溶液浸泡温石棉。
To explore the effects of several chemicals on chrysotileinduced cell proliferation in human embryo lung(HEL)cells,after soaking in aluminium citrate,mixed rare earths or sodium selenite solutions respectively at different concentrations for 1 hour,the chrysotile were incubated with HEL cells,then,the proliferation of HEL cells were determined by TdR incorporation assay.The results showed that chrysotile at concentrations of 25～10μg/ml with 24h action time,or at concentrations of 20～80μg/ml with 6h action time could promote the proliferation of HEL cells,but the chrysotile pretreated by these chemicals mentioned above only induced less cell proliferation.The results indicated that the carcinogenicity of chrysotile might be reduced by soaking with these compounds.
出处
《中国工业医学杂志》
CAS
1998年第2期70-72,共3页
Chinese Journal of Industrial Medicine
关键词
粉尘危害
石棉类
人胚肺细胞
细胞增殖
Asbestos,Human embryo lung cells (HEL),Cell proliferation
