摘要
目的:探讨HLA-DQA1基因多态性与宁夏回族人群类风湿性关节炎(RA)的相关关系。方法:采用多聚酶链反应/限制性片段长度多态性(PCR/RFLP)方法,对61例RA患者和81例健康对照者血样进行HLA-DQA1等位基因分析,寻找相关的基因型。结果:宁夏回族RA患者HLA-DQA1*0301(OR=32.45,P<0.01)和HLA-DQA1*0501(OR=3.11,P<0.01)等位基因显著高于宁夏回族健康人群;宁夏回族健康人群HLA-DQA1*0102(OR=0.19,P<0.01)和HLA-DQA1*0401(OR=0.04,P<0.01)等位基因显著高于宁夏回族RA患者。结论:HLA-DQA1*0301和*0501等位基因可作为宁夏回族RA易感基因的候选基因;HLA-DQA1*0102和*0401等位基因可作为宁夏回族RA保护基因的候选基因。
Objective To explore the relationship between alleles polymorphism of HLA-DQA1 gene and rheumatoid arthritis （RA） in Ningxia Huis Chinese. Methods Analysis of HLA-DQA1 gene was performed in 61 patients with RA and 81 healthy controls by polymerase chain reaction/restriction fragment length polymorphism （PCR-RFLP）. Results As compared to healthy controls, the frequency of HLA-DQA1^＊0301 （OR =- 32.45,P 〈 0.01） and HLA- DQA1^＊0501 （OR = 3.11 ,P 〈 0.01 ） alleles were higher, HLA-DQA1^＊0102 （OR = 0.19,P 〈 0.01 ） and HLA-DQA1^＊0401 （OR = 0.04,P 〈 0.01）alleles were lower in RA patients. Conclusion Alleles HLA-DQA1^＊0301 and ^＊0501 may be the susceptible genes of RA, alleles HLA-DQA1^＊0102 and ^＊0401 may be the protective genes of RA.
出处
《实用医学杂志》
CAS
北大核心
2009年第7期1055-1057,共3页
The Journal of Practical Medicine
基金
宁夏高校基金资助项目[编号:宁教高(2007)292]
关键词
关节炎
类风湿
聚合酶链反应
多态性
限制性片段长度
宁夏
回族
HLA-DQA1
Arthritis, rheumatoid
Polymerase chain reaction
Polymorphism, restriction fragment length
Ningxia
Hui nationality
HLA-DQA1 Gene
