摘要
目的制备粒径符合要求(200 nm以内)的反义寡核苷酸-聚丙交酯乙交酯纳米粒(ATM-ASODNs-PLGA-NP),并初步测定纳米粒的一些理化性质。方法以无毒的可生物降解的高分子材料聚丙交酯乙交酯作为载体材料,采用双乳化溶媒蒸发法制备载ATM-ASODNs-PLGA-NP,并评价其粒子形态、多分散性、包封率和进入人喉颈癌细胞(Hep-2)的能力等。结果制备的纳米粒形态圆整,大小均匀,平均粒径为87.9 nm,PDI为0.116,平均包封率为81.70%。经过PLGA纳米粒的包裹,对ATM-ASODN可起较好的保护作用,能载ATM-ASODN进入Hep-2细胞。结论载反义寡核苷酸纳米粒的制备工艺简便,粒子性状符合要求。
OBJECTIVE To study the technique for preparation of antisense oligodeoxynueleotide nanoparticles and evaluate the relevant characteristics of prepared nanoparticles. METHODS Biodegradable and biocompatible polymer polylactic co - glycolic acid （PLGA） was used to prepare ATM -ASODN loaded nanoparticles using double -emulsion evaporation technique. The characteristics of the nanoparticles, including size, PDI, encapsulation efficiency and the ability of transfer into Hep - 2 cells were evaluated. RESULTS The oligodeoxynucleotide nanoparticles we prepared had regular spherical surface and a narrow particle size with a mean diameter of 87.9 nm and PDI of 0. 116 . The average entrapment efficiency was 81.70%. The ATM - ASODN was protected from degradation by nucleases and was transferred into Hep -2 cells after being covered by PLGA nanoparticles. CONCLUSION The method for preparing oligodeoxynucleotide nanoparticles is simple and the properties of nanoparticles are conformed to the requirement of pharmaceutics.
出处
《华西药学杂志》
CAS
CSCD
北大核心
2009年第2期136-138,共3页
West China Journal of Pharmaceutical Sciences
关键词
反义寡核苷酸
聚丙交酯乙交酯纳米粒
双乳化溶媒蒸发法
包封率
细胞转染
Antisense oligodeoxynucleotide
PLGA nanoparticles
Double - emulsion evaporation technique
Entrapment efficiency
Cell transfer
