摘要
目的鉴别女宝胶囊中的当归和测定有效成分阿魏酸。方法采用TLC法鉴别4批女宝胶囊;用HPLC测定其中的阿魏酸,流动相为乙腈-0.085%磷酸溶液(17:83),检测波长320 nm。结果回归方程为:A=-9.02×104C+4.20×107(r=0.9988),阿魏酸的平均回收率为93.68%,RSD=1.04%(n=5)。结论所建方法简便、准确,重复性好,可用于女宝胶囊的质量控制。
OBJECTIVE To establish the identification methods for Angelica sinensis （Oliv. ） Diels and the determination method for ferulic acid in Nubao capsules. METHODS The TLC method was used to identify Angelica sinensis（ Oliv. ） Diels with test materitals and ferulic acid was determinated by HPLC. RESULTS The TLC specificities of 4 groups of Nubao capsule from 2 factories were proved. The HPLC used Symmetyshield^TM RP18 column as the analytical column. The mobile phase was aeetonitrile- 0. 085% phosphate acid solution（ 17 ： 83） and the detection wavelength was 320 nm. Regression equation was A = -9.02 × 10^4C ＋ 4.2 × 10^7 （r = 0. 9988 ）. The average recovery was 93.68% with RSD of 1.04% （ n = 5 ）. CONCLUSION The method is simple, accurate and reliable. It can be used for quality control of Nubao capsule.
出处
《华西药学杂志》
CAS
CSCD
北大核心
2009年第2期192-193,共2页
West China Journal of Pharmaceutical Sciences
