摘要
目的:研究β2肾上腺素受体阻滞剂对雌激素刺激大鼠成骨细胞分化与增殖的影响。方法:将不同浓度的β2肾上腺素受体阻滞剂(ICI118551)加入含雌激素(17β-E2)培养的第2代大鼠成骨细胞中,分别于培养3、6、9、12天时,使用PNPP法和放免方法检测成骨细胞培养基中的碱性磷酸酶(ALP)活性和骨钙素含量,并用MTT法检测成骨细胞的增殖率。结果:ICI118551可提高雌激素刺激成骨细胞培养上清中碱性磷酸酶浓度促进细胞增殖,尤其当其浓度为10-5及10-6mol/L时,但对成骨细胞培养上清中骨钙素水平的影响不显著。结论:β2肾上腺素受体阻滞剂能够显著促进雌激素刺激大鼠成骨细胞的释放和增殖作用。
Objective：To study the effects of β2AdR-blockers on the differentiation and proliferation of osteoblasts deprived from rats stimulated by estrogen. Methods：Put β2AdR-blockers at different concentrations into the second filter generation osteoblasts medium containing estrogen, after culturing 3,6,9,12 days, examining the alkaline phosphatase level of the osteoblasts by PNPP method and the content of the osteocalcin besides the growth rate of the osteoblasts by RI. Results ： The β2AdR-blocker increases the alkaline phosphatase （ALP） level and the level of growth rate of osteoblasts significantly, especially at the concentrations of 10^-5 and 10^-6 mol/L, but doesn＇t increase the content of osteocalcin. Conclusion：The β2AdR-blocker can enhance the dalivery and the proliferation of osteoblasts deprived from rats stimulated by estrogen significantly.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2009年第4期500-503,519,共5页
Journal of Nanjing Medical University(Natural Sciences)
