摘要
[目的]对蛹虫草产胞外多糖发酵培养进行研究。[方法]通过响应面方法分析蔗糖、蛋白胨和KH2PO43个主要因素对蛹虫草产胞外多糖得率的影响,以获得比较适宜的培养基组成。另外,对发酵培养的条件进行了研究。[结果]蛹虫草胞外多糖优化发酵培养基组成为:蔗糖2.00%,蛋白胨1.50%,KH2PO40.05%,酵母粉0.20%,硫酸镁0.01%;发酵培养的适宜条件为:pH值6.8,温度28℃。在上述条件下蛹虫草胞外多糖得率为19.4 g/L。[结论]得到蛹虫草产胞外多糖的优化工艺条件,为上罐提供理论依据。
[Objective] The research aimed to study the fermentation production of exo-polysaccharide by Cordyceps militaris.[Method] Through RSM the three major factors affecting the exo-polysaccharide production were analyzed,while the optimum medium and reaction conditions were obtained.The conditions of fermentation production were studied.[Result] The optimum media composition was as follows： Sucrose 2.00%,peptone 1.50%,KH2PO4 0.05%,yeast extract 0.20% and MgSO4 0.01%. The suitable fermentation condition was as follows： pH value 6.8, and the sture 28 ℃. At this condition, the exo-polysaccharide production rate was 19.4 g/L. [ Conclusion] The optimum process condition of exo-polysaccharide production by fermentation with Cordyceps militaris was obtained and offered theoretical basis for fermentor study.
出处
《安徽农业科学》
CAS
北大核心
2009年第9期3936-3938,3955,共4页
Journal of Anhui Agricultural Sciences
关键词
蛹虫草
胞外多糖
发酵
响应面
Cordyceps militaris
Exo-polysaccharide
Fermentation
RSM
