摘要
目的探讨丙型副伤寒、猪霍乱以及汤卜逊沙门菌的血清型鉴定要点,评价不同诊断血清的鉴定能力。方法用三家不同生产厂家的沙门菌诊断血清对丙型副伤寒、猪霍乱、猪霍乱库氏变种沙门菌三株标准株以及GSS中国区监测的疑似丙型副伤寒暴发分离株、猪霍乱和汤卜逊沙门菌进行血清鉴定,同时利用PCR、生化、以及MLST分型分析对这些菌株进行辅助分析。结果血清分型显示暴发菌株为汤卜逊沙门菌,那些原来鉴定为猪霍乱沙门菌的菌株也是汤卜逊沙门菌;PCR结果显示这些暴发菌株和散发菌株的Vi基因为阴性;还发现某些诊断血清的H:c因子血清能够与H:k抗原发生交叉凝集,从而导致血清型的误判;另外,MLST分型分析显示这些汤卜逊沙门菌菌株与丙型副伤寒、猪霍乱等沙门菌之间有差异;卫矛醇、粘液酸和H2S三种生化反应显示丙型副伤寒、猪霍乱、猪霍乱库氏变种沙门菌之间具有明显的生化差别。结论近期我国报告为丙型副伤寒的暴发中,需要鉴定是否为汤卜逊沙门菌所致,注意诊断血清的交叉反应所致的误判,可用生化鉴定、MLST分型分析以及PCR等方法进一步鉴定。
To investigate the differential criteria of S. thompson with S. paratyphi C and S. choleraesuis so as to evaluate the discriminating properties of various diagnostic sera, the serological identification of the reference strains of S. Paratyphi C. S. choleraesuis and S. choleraesuis var. Kunzendorf as well as the suspicious outbreak isolates of S. paratyphi C, S. choleraesuis and S. thompson in GSS of China was performed using the diagnostic antisera prepared by three different laboratories. Meanwhile, PCR assay, biochemical identification and MLST molecular typing were also carried out to assist the analysis, As demonstrated by the serotyping, the outbreak strain of salmonella was found to be S. Thompson and the strains of samonella originally identified as S. choleraesuis were proved to be S. thompson also. The Vi gene was absent in these strains as demonstrated by PCR assay. In addition, in some diagnostic antisera, the H：c antibody cross-reacted with the H2k antigen, thus making a wrong identification. The result obtained from MLST molecular typing showed that obvious genetic variations existed among Sal. thompson, Sal. paratyphi C and S. choleraesuis. The biochemical reactions of dulcitol, mucate and H2 S production also showed variations among S. paratyphi C. Sal. choleraesuis and S. eholeraesuis var. Kunzendorf. From the results of the above observation, it is apparent that the outbreak isolates identified as S. paratyphi C recently isolated in China should be re-analyzed to determine whether these isolates belong to S. thompson or to confirm the mis-identification due to the cross-reaction of diagnostic sera. Under this condition, the further identification using biochemical reactions, MLST molecular typing and PCR assay is necessary.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2009年第4期348-351,共4页
Chinese Journal of Zoonoses
