摘要
目的:建立反相高效液相色谱法(RP-HPLC)同时测定养坤丸中黄芩苷和芍药苷含量的方法。方法:采用Phenomenex Luna C18(250 mm×4.6 mm,5μm)色谱柱;流动相:乙腈-0.2%磷酸,梯度洗脱;流速:1.0 mL.min-1;检测波长:230nm;柱温:30℃。结果:黄芩苷与芍药苷能达到基线分离,线性良好。结论:该方法简便、快速、准确、重复性好,为养坤丸的质量控制提供了理论依据。
Objective： To develop an RP-HPLC method for the determination of baicalin and paeoniflorin in Yangkun Pills. Method： The sample was separated at 30℃ on a Phenomenex Luna Cls column （250 mm × 4.6 ram, 5μm） eluted with acetonitrile and water containing 0.2 % Phosphoric acid in gradient mode. The detection wavelength was 230 nm, and the flow rate was 1.0 mL·min^-1 . Result： Two compounds were baseline-isolated, the linear relationships of the injection amounts and peak areas for both baicalin and paeoniflorin were excellent. Conclusion： The method was simple, rapid, precise and repeatable. This method can be used for controlling the quality of Yangkun Pills.
出处
《中国实验方剂学杂志》
CAS
北大核心
2009年第4期12-14,共3页
Chinese Journal of Experimental Traditional Medical Formulae
