摘要
目的研究单次与多次经静脉移植骨髓间充质干细胞（MSCs）对大鼠急性心肌梗死的影响。方法选择60只SD大鼠随机分为A组（单次移植MSCs）、B组（多次移植MSCs）、C组（单次移植MSCs对照）和D组（多次移植MSCs对照），每组15只。A组在心肌梗死后第1天、B组在心肌梗死后第1、4、7、10、13天经尾静脉移植MSCs5×10^6个，C组和D组注射等量培养液。大鼠心肌梗死后4周测定左心功能，并行免疫组织化学检测。结果心肌梗死后4周A组和B组大鼠梗死心肌中均可以观察到4’，6二脒基-2-苯吲哚标记阳性细胞存在。与c组和D组比较，A组和B组大鼠左心功能均明显改善，心肌梗死面积减小，毛细血管密度增高（P〈0．05）。与A组比较，B组改善作用更加明显（P〈0．05）。结论经静脉单次与多次移植Mscs对大鼠急性心肌梗死均有效，但多次移植效果更好。
Objective To investigate the effect of intravenous transplantation of bone marrow mesenchymal stern cells（MSCs） for one time and many times on acute myocardial infarction（AMI） in rats. Methods Sixty rats were randomly divided into group A（iv transplantation for one time）, group B（iv transplantation for many times）,group C（control of iv transplantation for one time） and group D（control of iv transplantation for many times） of 15 rats each. 5 × 10^6 labeled MSCs were injected to AMI rats on day 1（group A） and days 1,4,7,10,13（group B） after MI,and culture medium of the same volume was injected to groups C and D. Four weeks after MI,the cardiac function was evaluated and immunohistochemical analysis was performed. Results Many DAPI positive cells could be found in infarcted myocardium of groups A and B. Compared with groups C and D,the left ventricular function was obviously improved and capillary density was obviously increased while infarct size＇was significantly reduced in groups A and B （P 〈0.05）. There was significant difference between＇groups A and B （P 〈0.05）. Conclusion The results demonstrated that iv transplantation of bone marrow mesenchymal stem cells for many times has better effect on rats with AMI compared with transplantation for one time.
出处
《中华老年心脑血管病杂志》
CAS
北大核心
2009年第4期278-280,共3页
Chinese Journal of Geriatric Heart,Brain and Vessel Diseases
基金
安徽省教育厅自然科学重点基金(2006KJ088A)
关键词
间质干细胞移植
静脉
心肌梗塞
骨髓移植
mesenchymal stem cell transplantation
veins
myocardial infarction
bone marrow transplantation
