摘要
利用电化学生长法制备了纳米银DNA修饰电极。在pH4．1的磷酸盐（PBS）缓冲溶液中研究了抗坏血酸（AA）在该电极上的电化学行为，实验得到电荷传递系数α=0．41、扩散系数D=1．22×10^-5cm^2／s。建立了利用修饰电极催化作用快速测定抗坏血酸的方法，修饰电极对抗坏血酸的催化氧化峰与抗坏血酸的浓度分别在1．0×10^-7～5．0×10^-5mol／L、5．0×10^-6～5．0×10^-8mol／L范围内呈线性关系，检出限为1．0×10^-9mol／L。该方法快速、灵敏。
Silver nanoparticle and DNA have been modified on gold electrode by a new method according to the electrochemical-assemble principle. Electrochemical properties of ascobic acid on the electrode was characterized in PBS buffer solution （pH = 4. 1）. Some important electrochemical kinetic parameters including charge transfer coefficient （a=0.41） and diffusion coefficient （D= 1. 22 ×10^-5cm2/s） were calculated. The linear relationship was observed between the oxidation peak current and ascorbic acid concentration in range of 1×10^-2～5×10^-5mol/Land 5×10^-6～5×10^-8mol/L, respectively with the detection limit of 1×10^-9mol/L.
出处
《分析科学学报》
CAS
CSCD
北大核心
2009年第2期169-172,共4页
Journal of Analytical Science
关键词
DNA
抗坏血酸
纳米银
电化学行为
DNA
Ascorbic acid
Silver nanoparticle
Electrochemical behavior
