摘要
In HCl-NaOAc of pH 4.30 buffer medium,pioglitazone hydrochloride reacts with eosin Y to form an ion-association complex,and the colour of solutions change obviously at the maximum fading wavelength 516 nm.The fading colour intensity is also proportional to the concentration of pioglitazone hydrochloride,and a sensitive spectrophotometric method is developed for determination of pioglitazone hydrochloride.The concentration of pioglitazone hydrochloride in the range of 7.05×10-7 mol/L～2.48×10-5 mol/L obeys Beer’ law at the maximum fading wavelength 516 nm.The apparent molar absorptivity ε516 is 3.65×104 L·mol-1·cm-1,and the detection limit is 3.16×10-7 mol/L.The method has high sensitivity and fairly good selectivity,and was applied to the determination of pioglitazone hydrochloride in medicine samples and human serum and human urine with satisfactory results.
In HCl-NaOAc of pH 4.30 buffer medium, pioglitazone hydrochloride reacts with eosin Y to form an ion-association complex, and the colour of solutions change obviously at the maximum fading wavelength 516 nm. The fading colour intensity is also proportional to the concentration of pioglitazone hydrochlofide, and a sensitive spectrophotometric method is developed for determination of pioglitazone hydrochloride. The concentration of pioghtazone hydrochloride in the range of 7.05 × 10^-7 mol/L - 2.48 × 10^-5 mol/L obeys Beer＇ law at the maximum fading wavelength 516 nm. The apparent molar absorptivity ε516 is 3.65 × 10^4 L ·mol^-1 · cm^-1 ,and the detection lim/t is 3.16× 10^-7 mol/L. The method has high sensitivity and fairly good selectivity, and was applied to the determination of pioglitazone hydrochloride in medicine samples and human serum and human urine with satisfactory results.
出处
《化学研究与应用》
CAS
CSCD
北大核心
2009年第4期542-545,共4页
Chemical Research and Application
基金
重庆市教委科研项目(KJ081301)
第二批"重庆市高等学校优秀中青年骨干教师资助计划"[渝教人(2005)2号]
长江师范学院科研项目(07Jkg50)
关键词
分光光度法
盐酸吡格列酮
曙红Y
speetrophotometry
pioglitazone hydrochloride
eosin Y
