摘要
目的探讨s-油酰丙醇胺对用肿瘤坏死因子α（TNF—α）诱导的人脐静脉内皮细胞黏附分子（VCAM-1，I-CAM-1，E选择素）表达的影响。方法从新鲜的脐带中分离出人脐静脉内皮细胞，培养至3～9代，用不同浓度的s-油酰丙醇胺（10，50，100μmol／L）孵育12h后，用TNF-α（20ng／mL）孵育8h，采用荧光实时定量PCR和细胞酶联免疫吸附试验分别检测VCAM-1，ICAM-1，E选择素的mRNA及蛋白的表达，同时采用细胞黏附实验检测其对细胞黏附的影响。结果相对于正常的人脐静脉内皮细胞，TNF—α诱导后的人脐静脉内皮细胞黏附分子（VCAM-1，ICAM-1，E-选择素）的表达明显增加。s-油酰丙醇胺可以显著的抑制VCAM-1的表达，并呈现出一定的剂量依赖性，而且对人急性单核细胞性白血病细胞（THP-1）的黏附也有明显的抑制作用，但对ICAM-1，E-选择素的表达却没有影响。结论s-油酰丙醇胺和大多数的PPARα激动剂一样，能够抑制慢性炎症，减少单核细胞的黏附，抑制VCAM-1的表达，而对急性炎症没有作用，如对E-选择素的表达无影响。
Purpose To investigate the effect of s-Oleylpropanolamide on the expression of cell adhesion molecules such as VCAM-1, ICAM-1, E-selectin in human umbilical vein endothelial cells（HUVECs）. Meth- ods HUVECs were pretreated with different concentrations of s-Oleylpropanolamide for 12 h and then stimulated with TNF-α for 8 h. Cell Enzyme linked immunsorbendt assay. Real-time PCR and adhesion assay were performe to measure the expression of ahesion molecules in mRNA and protein levels and monocyte binding. Resuits s-Oleoylpropanolamide inhibited monoeyte binding and the expression of VCAM-1,an adhesion molecule relatively specific for monocyte and lymphocyte adhesion and chronic inflammation. However it showed no effect on the expression of E-selectin nor ICAM-1. Conclusion s-Oleoylpropanolamide has the same effect as most of PPARa activitors on inhibiting the development of atherosclerosis.
出处
《中国生化药物杂志》
CAS
CSCD
北大核心
2009年第2期99-102,共4页
Chinese Journal of Biochemical Pharmaceutics
基金
厦门市科技计划高校创新项目(3502Z20083007)
厦门大学"活性有机小分子的合成化学与化学生物学"创新团队项目
