摘要
建立了毛细管电泳电化学法检测尿样中苯丙胺的方法。以直径33μm的碳纤维电极为工作电极，在最佳检测条件即检测电位1．30V，15kV下电动进样3s，选择电泳分离高压为15kV，电泳缓冲液为pH10．0的20mmol／L的磷酸盐，实验发现，在1．0×10^-81．0×10^-5mol／L范围内，响应电流与苯丙胺浓度呈良好的线性关系，线性相关系数为0．9984，检出限达3．3×10^-9mol／L。对于浓度为1．0×10^-5mol／L的苯丙胺，峰电流及迁移时间的RSD分别为2．4％和2．5％（n=7）。对于尿样中2．0×10^-5mol／L的苯丙胺，回收率为75％。
A novel method was developed for the determination of amphetamine （AM） by capillary electrophoresiscoupled with electrochemical detection（ CE- ECD）. The effect of detection potential, concentration and pH value of running buffer on the peak current of AM was investigated and the optimal conditions were obtained as follows ： detection potential, 1.30 V ; electrokinetic injection, 15 kV for 3 s; separation voltage, 15 kV; running buffer： 20 mmol/L pH 10. 0 phosphate buffer. Under the optimal conditions, the peak currents exhibits a good linearity with concentrations of amphetamine in the range of 1.0 × 10^-8 -1.0 × 10^-5 mol/L with a correlation coefficient of 0. 998 4. The detect limit was down to 3.3 × 10^-9 mol/L. RSDs（n =7） of peak current and migration time of 1.0 × 10^-5 mol/L AM were 2. 4% and 2. 5% , respectively. A recovery of 2.0 × 10^-5 mol/L AM spiked in urine blank sample was 75% . The result obtained indicated that the method was rapid and sensitive, and could be applied in the determination of AM in real urine sample.
出处
《分析测试学报》
CAS
CSCD
北大核心
2009年第4期474-477,共4页
Journal of Instrumental Analysis
基金
国家自然科学基金资助项目(20605020)
关键词
毛细管电泳
电化学检测
苯丙胺
capillary electrophoresis
electrochemical detection
amphetamine
