摘要
以菜籽清蛋白为原料,利用碱性蛋白酶和木瓜蛋白酶对其进行双酶分步水解。在单因素实验的基础上,采用正交实验对水解条件进行优化。结果表明:在最适底物浓度3.5%条件下,先用4000U/g碱性蛋白酶于pH8.0、55℃下水解3h后,再用3000U/g木瓜蛋白酶于pH5.5、50℃下水解3h,菜籽清蛋白的水解度达到40.17%,氮溶解指数为90.89%。
Rapeseed albumin was used as material and the parameters of step enzymic hydrolysis of alcalase and papain were studied. On the single-factor experiments, the optimum conditions of alcalase and papain were studied by orthogonal experiments. Rapeseed albumin of 3.5% concentration was hydrolyzed by 4000U/g of alcalase at 8.0 of pH and 55℃ for 3h and then by 3000U/g of papain at 5.5 of pH and 50℃ for 3h.The degree of hydrolysate was 40.17 %.The nitrogen solution index of the hydrolysate was 90.89%.
出处
《食品工业科技》
CAS
CSCD
北大核心
2009年第4期149-152,共4页
Science and Technology of Food Industry
基金
国家"十一五"科技支撑计划项目(2006BAD05A12)
