摘要
本研究分离了一株鹅I型副粘病毒(ZQ042),应用RT-PCR技术对其编码的核蛋白(NP)、磷蛋白(P)、基质蛋白(M)、血凝素-神经氨酸酶蛋白(HN)、融合蛋白(F)和巨蛋白(L)6种主要结构蛋白分别进行扩增,然后将其克隆到pMD18-T载体后进行序列测定和拼接;并将克隆到的6个基因片段与国内外已报道的I型副粘病毒基因序列进行比较分析。分析结果表明,鹅副粘病毒分离株ZQ042F基因与La Sota株同源性最高,同源率为99.1%,与其它各毒株各基因同源性均较低。
Reverse transcription polymerase chain reaction （RT-PCR） was used to amplify the six major encoding structure protein of an A goose paramyxovirus type Ⅰ （ZQ042）. The cDNAs were then cloned to pMD18-T vector and sequenced. These sequences were compared with paramyxovirus type Ⅰ home and abroad reported. The results showed that the F gene was comparatively far related with La Sota. rate of homologous was 99.1%, and other genetic homology of the strains was low.
出处
《中国畜牧兽医》
CAS
北大核心
2009年第4期80-86,共7页
China Animal Husbandry & Veterinary Medicine
