摘要
对一株分离自深海热液口古菌HJ21进行了分类鉴定及高温酶活性的研究。该菌株是极端嗜热的厌氧球菌，直径为1．0～1．2μm。菌株最适生长温度88℃；菌株生长pH为5．0—9．0，最适pH为6．5～7．0；菌株生长NaCI质量浓度为10～50g·L^-1，最适为20g·L^-1。根据其形态特征、生理生化特性以及16S rRNA基因序列分析结果，确定HJ21菌株为热球菌属（Thermococcus）。该菌株能产生高热稳定的高温α-淀粉酶、普鲁兰酶、α-葡萄糖苷酶和蛋白酶，这些酶的最适作用温度分别为95、95、100和100℃，α-淀粉酶、普鲁兰酶和α-葡萄糖苷酶在90℃的半衰期分别为5、5和2h；蛋白酶在100℃保温2．5h后仍具有84％的酶活性。
The identification and hyperthermophilic enzyme activities of an archaeon from a deep-sea hydrothermal vent were studied. The extremely thermophilic anaerobic archaeon strain H J21 was irregular cocci with 1.0 - 1.2 μm in diameter. The optimum temperature of growth was 88 ℃. The pH range for growth was 5.0 -9. 0, with an optimum pH of 6. 5 -7.0. The NaCI concentration range for growth was 10 -50 g · L^-1, with an optimum concentration of 20 g · L^-1. It belonged to the genus Thermococcus based on the 16S rRNA gene sequence and morphological, biochemical and physiological characteristics. The strain HJ21 produced thermostable eL-amylase, pullulanase, α-glucosidase and protease. The optimal working temperatures of the α-amylase, pallulanase, α-glucosidase and protease were 95, 95, 100 and 100℃, respectively. The half-life of the a-amylase, pullulanase, α- glueosidase were 5, 5 and 2 h at 90 ℃ , respectively; 84% of the protease remained active after incubation at 100℃ for 2. 5 h.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2009年第2期130-136,共7页
Journal of Nanjing Agricultural University
基金
国家自然科学基金项目(40746030)
江苏省“六大人才高峰”第三批资助项目(06-A-017)
江苏省教育厅自然科学基金项目(06KJB550004)
江苏省海洋生物技术重点建设实验室项目(2006HS008)
连云港市自然科学基金项目(KK06076)
