摘要
目的建立桂枝茯苓软胶囊中芍药苷的含量测定方法。方法采用D-101大孔吸附树脂对芍药苷进行分离纯化。采用反相高效液相色谱法测定芍药苷的含量,色谱柱:Agilent TC-C18柱(5μm,250mm×4.6mm);流动相:KH2PO4(0.02mol/L pH=3.0)∶乙腈=83∶17;检测波长:232nm;流速:1.2mL.min-1;柱温:30℃;进样量5μl。结果芍药苷在4μg/mL-28μg/mL的范围内线性关系良好(r=0.9999),RSD=0.13%(n=6),检测限为0.02μg/mL,样品的平均回收率为92.15%。结论该方法简便,灵敏,重现性好,可作为桂枝茯苓软胶囊中芍药苷的含量测定方法。
Objective To establish a RP-HPLC method for determining the content of paeoniflorin in guizhi fuling soft capsule. Methods The extraction and purification of paeoniflorin was performed with D-101 maeroporeus absorbed resin . The content of paeoniflorin was determined by RP-HPLC with the following materials and parameters： agilent TC-C18 column as the separating column, buffer con- sisted of KH2PO4 （0.02mol/l, pH = 3.0） and aeetonitrile （83：17, v/v） as the mobile phase, 1.2 ml/min as the flow rate, 232nm as the detection wave length, 30℃ as the column temperature, and 5 μl as the injection volume. Results The compounds were well separated. The calibration curves of paeoniflorin in the range of （4μg/ml- 28μg/ml） were linear. The lowest detectable concentration of paeoniflorin was 0.02μg/m]. The RSD was less than 0.13%. The average recoveries were 92.15 %. Conclusion The method is simple, sensitive and precise. It could be used for the determination of paeoniflorin in guizhi fuling soft capsule.
出处
《川北医学院学报》
CAS
2009年第2期121-123,共3页
Journal of North Sichuan Medical College
