摘要
目的观察重组人促红细胞生成素rh-EPO对大鼠脑损伤后皮层脑红蛋白NGB表达的影响,探讨其在脑损伤后的意义。方法78只大鼠随机分为3组:假手术组6只,单纯颅脑损伤组36只,rh-EPO干预组36只。单纯颅脑损伤组与rh-EPO干预组模型分别按照3h、6h、12h、24h、48h、72h6个时间点随机分为6个亚组,每个亚组6只大鼠,通过免疫组化及图像分析方法,观察打击区周边皮层中脑红蛋白NGB的表达。结果单纯颅脑损伤组打击周边区NGB蛋白免疫反应阳性细胞表达较假手术组增多(P<0.01),在相应区域rh-EPO干预组明显高于前两组(P<0.01)。结论rh-EPO可诱导增加大鼠脑损伤后脑红蛋白的表达。
Objective To observe the effects of recombinant human erythropoietin （rh-EPO） on neuroglobin （NGB） expression in cerebral cortex and explore its significance after traumatic brain injury in rats. Methods A total of 78 rats were divided into three groups： sham group （n =6）, traumatic brain injury （TBI） group （n = 36） and rh-EPO group （n = 36）. TBI group and rh-EPO group were divided into 6 subgroups respectively at 3, 6, 12, 24, 48 and 72 h post injury, with 6 rats in each group. NGB expression in the penumbra zone of traumatic cortical lesion was studied by immunohistochemical staining and computer-assisted image analysis. Results The expression of neuroglobin immune response （NGB-IR） in penumbra zone of traumatic cortical lesion in TBI group was more than that in sham group. The expression of NGB in the same area was the highest in rh-EPO group （P 〈 0.05）. Conclusion rh-EPO can induce the expression of NGB in cerebral cortex after brain injury.
出处
《中华神经外科疾病研究杂志》
CAS
2009年第2期101-104,共4页
Chinese Journal of Neurosurgical Disease Research
基金
全军十一五医学基金资助项目(06G032)
