摘要
目的探讨复方甘草酸苷对哮喘大鼠气道炎症的影响及其机制。方法40只SPF级Wistar大鼠随机分成健康对照组（Ⅰ组）、哮喘模型组（Ⅱ组）、复方甘草酸苷哮喘治疗组（Ⅲ组）、哮喘治疗阴性对照组（Ⅳ组），10只／组，Ⅱ、Ⅲ、Ⅳ组大鼠用卵清蛋白（OVA）氢氧化铝混合液腹腔注射致敏和OVA超声雾化吸入激发建立哮喘大鼠模型，Ⅲ组大鼠每次激发前1h腹腔注射复方甘草酸苷200μg，Ⅳ组大鼠每次激发前用等体积生理盐水代替复方甘草酸苷腹腔注射，Ⅰ组大鼠腹腔注射用等体积生理盐水代替OVA氢氧化铝混合液，超声雾化吸入用等体积生理盐水代替OVA。所有大鼠在末次激发后6h取中心静脉血2ml测定IgE含量，取左肺支气管肺泡灌洗液（BALF）行细胞计数与分类，并测定BALF中细胞因子IL4、IL-5、IL-13及IFN-γ含量。结果Ⅱ组静脉血IgE含量及BALF中中性粒细胞、淋巴细胞、嗜酸性粒细胞计数和IL-4、IL-5、IL-13含量显著高于Ⅰ组和Ⅲ组（P〈0．01），IFN-γ含量显著低于Ⅰ组和Ⅲ组（P〈0．01）；Ⅱ组与Ⅳ组各项指标差异均无统计学意义（P〉0．05）；Ⅲ组与Ⅰ组相比，IgE含量及中性粒细胞、淋巴细胞、嗜酸性粒细胞计数和IL4、IL-5及IL-13含量升高，单核巨噬细胞计数和IFN-γ含量降低，但均无统计学意义（P〉0．05）。结论复方甘草酸苷可能通过调节Th1／Th2细胞因子平衡，来抑制Th2细胞免疫学效应，促进Th1细胞免疫学效应，从而减轻气道炎症，降低气道高反应性。
Objective To investigate the effect and mechanism of compound glyeyrrhizin on airway inflammation in asthmatic rats. Methods Forty wistar rats were randomly divided into health group （groupⅠ） , asthma group （group Ⅱ） , treating group （group Ⅲ ） and treating control group （ group Ⅳ） （ n = 10 in each group）. Group Ⅱ, group Ⅲ and group Ⅳ received intraperitoneal injection of ovalbumin （OVA） and aluminum hydroxide gel for sensitization and OVA aerosol by ultrasonic nebulization for challenge, in addition, group Ⅲ received intraperioneal injection of 200 txg of compound glycyrrhizin 1 hour before OVA aerosol challenging every time, and group IV were injected with normal saline instead of compound glycyrrhizin. Group Ⅰ were injected and nebulizated with normal saline instead of OVA and aluminlum hydroxide. All rats were sacrificed at the 6th hour after the last challenging, venous blood and bronchoalveolar lavage fluid（BALF） were collected, the IgE in venous blood were determined and the concentration of IL-4, IL-5, IL-13 and IFN-γ in BALF were detected, the cells in BALF were counted and detached. Results The concentration of IgE, IL-4, IL-5, IL-13 and the count of neutrophilic granuloeytes, lymphoeytes, eosinophils in group II were significantly higher than those in group Ⅰ and group Ⅲ（ P 〈 0. 01 ） , and the concentration of IFN- γ in group Ⅰ and group IV were significantly lower than those in group I and group Ⅲ（P〈0.01）. There was no significant difference between group Ⅱ and group Ⅳ（ P〉0. 05）. The concentration of IgE, IL-4, IL-5, IL-13 and the count of neutrophilic granulocytes, lymphocytes, eosinophils in group Ⅲ were higher than those in group I, and the concentration of IFN-γ and the count of mononucleat macroplile cells in group III were lower than those in group Ⅰ, but there were no significant difference （ P 〉 0. 05 ）. Conclusion Compound glycyrrhizin alleviated the airway inflammation and reduced the airway hyperresponsiveness in asthmatic rats, which mechanism may be related to regulating the balance of Th1/Th2 cytokines, suppressing the immune ability of Th2 and promoting the immune ability of Th1.
出处
《中国医师杂志》
CAS
2009年第4期491-493,共3页
Journal of Chinese Physician
