摘要
目的探讨Adv-mIL-12转染KC对人结直肠癌细胞的生长抑制作用。方法腺病毒Adv-mIL-12体外转染KC,ELISA法检测其IL-12的表达;将KC,KC-Adr-EGFP和KC-Adv-mIL-12与LoVo人结直肠癌细胞共培养,MTT和ELISA法检测其对LoVo细胞的生长抑制率和VEGF表达量的影响;FCM检测不同KC组的上清液诱导LoVo细胞凋亡的差异。结果KC-Adv-mIL-12中IL-12浓度明显提高[(564.29±31.24)pg/mL24h];MTT,ELISA和FCM等证实KC-Adv-mIL-12对LoVo细胞的抑制作用较另2个对照组明显(P<0.05),LoVo细胞的凋亡率显著增加。结论mIL-12基因转染KC能有效抑制人结直肠癌细胞的生长及转移。
Objective To explore the effect of Kupffer Cells （ KC ） transfected with a recombinant adenoviral vector expressing interleukin 12 （IL-12 ） on human coloreetal cancer LoVo cells. Methods ELISA method was used to detect quantitatively the level of IL-12 of Kupffer cells transfected with Adv-mIL-12. KC, KC-adv-EGFP and KC-Adr-mIL-12 with LoVo cells were cultured in different plates. The cells viability and the supernatants＇ VEGF were measured by MTT assay and VEGF ELISA kit;different supernatants groups of LoVo cells were harvested and differences of apoptosis were analyzed using FCM. Results A high level of secretion of IL-12 was detected in the adv-mIL-12-transfeeted KC [ （564.29±31.24 ）pg/mL 24 h]. The MTT assay and ELISA test showed that the inhibition ratio of LoVo cells was higher in the experimental group compared with 2 control groups （ P 〈 0.05 ） ; the FCM showed that more apoptosis cells were detected in the the experimental group （21% ）. Conclusions KC-Adv-mIL-12 can inhibit the proliferation and metastasis of LoVo cells.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2009年第4期358-361,共4页
China Journal of General Surgery
