摘要
目的：为研究日本血吸虫（中国大陆株）22．6kDa抗原的免疫保护性。方法：将其编码cDNA经PCR扩增后亚克隆入载体质粒PGEX—1λt进行表达，并用重组抗原免疫了一批昆明鼠。结果：与对照组相比，重组抗原免疫小鼠获得了38．93％的减虫率。结论：证明重组的日本血吸虫22．6kDa抗原可诱导宿主抗血吸虫感染的部分保护力。
To evaluate the protective level of Schistosoma japonicum 22.6kDa antigen,a cDNA fragment,encoding Sj22.6kDa antigen, was amplified from the clone Sj 514 by PCR andsubcoloned into expression plasmid vectorpGEX-IXT which coded for 22.6kDa GST of S. japonicum,and then transferred into E. coli TG2. The positiverecombinant poasmid could express 22.6kDa protein as well as 26kDa GST in E. coli TG2. The positive recombinant plasmid could express 22.6kDa protein as well as 26kDa GST in E. coli TGZ after being induced by IPTG.But the two proteim were expressed separately. The sequence data showed that there was a stop coden TAA inthe upstream of the start coden ATG of the open reading frame,which might explain why 22.6kDa proten and26kDa GST were not expressed in fusion protein.Mice were vaccinated with recombinant 22.6kDa antigen in the band cut from SDS-PAGE gel and mixedwith FCA (Complete Freunds ad invent). Vaccination experiments showed that there was a protection,about 38.93%,in terms of worm burden reduction,but no significant level of reduction in the number of eggs in the live ofimmunized mice. Recombinant Sj 22.6kDa antigen was a promising vaccine candidate of S. japonica.
出处
《中国人兽共患病杂志》
CSCD
北大核心
1998年第2期11-14,共4页
Chinese Journal of Zoonoses
基金
本课题获总理专项基金!(94-Y-23)
