摘要
在生理pH=7.4环境下,用摩尔比法测定γ-环糊精(γ-CD)与达旦黄(TY)的包合比(摩尔比)nγ-CD:nTY=1:1,直线法测定包合常数Kf=495.23L/mol。以中性红(NR)作分子探针,用紫外光谱法、荧光光谱法、电化学法、化学热力学法和黏度法等研究包合物γ-CD-TY与鲱鱼精DNA的作用,得到γ-CD-TY包合物与鲱鱼精DNA作用的结合比nγ-CD-TY:nDNA=6;1,结合常数为K2Θ98.15K=1.12×105L/mol,K3Θ10.15K=2.72×105L/mol。热力学函数ΔrGmΘ=-2.82×104J/mol,ΔrHmΘ=5.67×104J/mol,ΔrSmΘ=284.78J/(mol·K),说明γ-CD-TY包合物与DNA作用为熵驱动。确定γ-CD-TY与鲱鱼精DNA的作用方式为混合作用,同时存在部分嵌插和非嵌插作用方式。
Under the condition of physiological environment pH 7.4, the binding ratio nγ-CD：^nTY=1：1 and binding constant Kf=495.23 L/mol were confirmed by mole ratio method and linear method. NR was used as molecule probe, and the interaction between γ-CD-TY and herring-sperm DNA was studied by UV spectra, fluorescence spectra, electrochemistry, thermodynamic method and viscosity method, etc. A series of thermodynamic functions such as △rGm^⊙=-2.82×10^4J/mol, △rHm^⊙=5.67×10^4J/mol, △rSm^⊙=284.78J/（mol·K） were obtained, and △rGm^⊙ is the primary driven power of this o interaction, the binding ratio is nγ-CD-TY^nDNA=6：1, the binding constants are K298.15K^⊙=1.12×10^5L/mol, K310.15K^⊙=2.72×10^5L/mol. The interaction mode between γ-CD-TY and herring-sperm DNA is a mixed binding, consisting of partly intercalation and dis-intercalation.
出处
《中南大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2009年第2期367-374,共8页
Journal of Central South University:Science and Technology
基金
国家自然科学基金资助项目(30572254)
关键词
Γ-环糊精
达旦黄
包合物
鲱鱼精DNA
作用方式
γ-cyclodextrin
titan yellow
inclusion complex
herring-sperm DNA
interaction mode
