摘要
目的:探讨硫化氢(H2S)对兔左心室心肌细胞电生理特性的影响。方法:用标准微电极细胞内记录技术,观测硫化氢对兔左心室心肌细胞电生理特性的影响。观测指标:静息电位(resting potential,RP)、超射值(over-shoot,OS)、动作电位幅值(amplitude of action po-tential,APA)、0相最大除极速率(maxi mal rate ofdepolarization,Vmax)、复极20%、50%和90%时间(20%、50%and90%of duration of action potential,APD20、APD50and APD90)及复极化平均速度(average rate of repolarization)。结果:(1)用50μmol/L NaHS灌流时,和正常对照组相比,APD50、APD90缩短(P<0.01),复极化平均速度增快(P<0.05)。(2)100μmol/L NaHS与对照组比较,APD50、APD90缩短(P<0.01),复极化平均速度增快(P<0.05)。和50μmol/L NaHS相比APD50缩短(P<0.01)、APD90缩短(P<0.05)、复极化平均速度增快(P<0.05)。(3)200μmol/L NaHS、400μmol/L、1000μmol/L NaHS可浓度依赖性的使APA、RP、OS减小(P<0.01),APD20、APD50、APD90缩短(P<0.01),复极化平均速度增快(P<0.01)。结论:H2S可浓度依赖性的影响兔左心室心肌细胞电生理特性。
Objective：To investigate the effects of hydrogen sulfide（H2S）on action potentials（Ap）of rabbit left ventricular myocytes. Methods： By using standard intracellular microelectrode technique to record Ap, hydrogen sulfide was used to investigate the electrophysiological features of left ventricular myocytes. Elec- trophysiological parameters examined were：RP（resting potential）, APA（amplitude of action potential）, Vmax （maximal rate of depolarization） ,OS（over-shoot） ,APD20, APD50 and APD90 （20%, 50% and 90% of duration of action potential）and average rate of repolarization. Results： （1）Perfusion with NariS（ H2 S donor, 50μmol/L） led to a significant decrease in APD50 （P〈0. 01）, APD90 （P〈0. 01）and a significant increase in average rate Of repolarization（P〈0.05）. （2）Application of 100/μmol/L Naris resulted in a significant shortening in APD50（P 〈0. 01）, APD90 （ P 〈 0. 01 ） and a significant increase in average rate of repolarization （ P 〈 0.05）. Compared with the result of 50μmol/L NariS, 100μmol/L NariS led to a significant shortening in APD50 （P〈0.01）, APD90 （P〈0. 05）and a significant increase appeared in average rate of repolarization（P〈0. 05）. （3）Application of 200μmol/L,400μmol/L and 1 000μmol/L NariS led to a significant decrease in APA,RP and OS,a signifi- cant shortening in APD20, APD50 and APD90, a significant increase in average rate of repolarization in a concen- tration-depedent manner. Conclusion： H2 S could significantly affect the electrophysiology of guinea pig ventricu- lar myocytes in a concentration-depedent manner.
出处
《河北北方学院学报（医学版）》
2009年第2期7-11,共5页
Journal of Hebei North University:Medical Edition
基金
河北省教育厅自然科学研究项目(编号:2007104)
河北北方学院校基金资助项目
关键词
电生理学
硫化氢
肌细胞/心脏
Electrophysiology, Hydrogen Sulfide, Myocytes/Cardiac
