摘要
建立了用高效液相色谱-紫外检测法检测禽蛋及其制品中三聚氰胺的方法。采用乙腈作为样品中蛋白质的沉淀剂和三聚氰胺提取剂,流动相为0.05mol/L(pH=3.00)的磷酸二氢钾乙腈缓冲溶液,检测波长为240nm,流速为1.50mL/min,SCX(Luna 5u SCX250×4.6mm)色谱柱进行分离,HPLC-UV检测,外标法定量。结果表明,该方法简便快捷,线性范围0.20～10.00mg/L,回收率在99.8%-109.4%,线性关系良好R2=1.000,可广泛应用于禽蛋及其制品中三聚氰胺的检测。
A method for determination of melamine in eggs and eggs products by HPLC-UV was established. Melamine in eggs was extracted and the protein in samples was precipitated with acetronitrile,melamine was separated on a Luna SCX column （Luna 5u SCX 250 × 4.6mm） with monopotassium phosphate （MKP 0.05mol/L,pH=3.00）,acetronitrile buffer solution（70：30,by volume） as the mobile phase at flow rate 1.50mL/min and the detection wavelength was 240nm,quantitative with external standard method.the linear ranges were 0.20 - 10.00mg/L, the melamine revoveries were in the range of 99.8% - 109.4 %, and the related coefficient was 1.000. The method was proved to be simple and accurate,and has been applied to determine melamine in eggs and egg products.
出处
《福建分析测试》
CAS
2009年第2期55-58,共4页
Fujian Analysis & Testing
关键词
三聚氰胺
禽蛋
高效液相色谱-紫外
检测
melamine
Eggs
high performance liquid chromatography（HPLC）-UV
determination
