摘要
目的探讨铝螯合剂1,2-二甲基-3-羟基-4-吡啶酮(1,2-dimethyl-3-hydroxy-4-pyridone,DFP)对染铝大鼠睾丸组织中抗氧化系统及铝、铁、锌、钙、镁、铜元素的影响。方法将35只健康清洁级雄性Wistar大鼠随机分为5组,分别为阴性对照组(生理盐水)、低、中、高剂量DFP+Al组和阳性对照组。前8周,低、中、高剂量DFP+Al组和阳性对照组均给予172.5mg/(kg·d)的氯化铝溶液。后3周,阳性对照组给予1ml生理盐水,低、中、高剂量DFP+Al组分别给予剂量为13.82、27.44和54.88mg/(kg·d)的DFP溶液。给药均采用灌胃方式染毒,每周连续5d,间隔2d后继续给药。染毒后24h,取出睾丸,称重,并计算睾丸系数。测定各组睾丸组织中超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量以及铝、铁、锌、钙、镁、铜元素的含量。结果各组大鼠体重、睾丸重量和睾丸系数间比较,差异均无统计学意义(P>0.05)。随着DFP染毒剂量的升高,睾丸组织中SOD活力呈上升趋势,MDA含量呈下降趋势。与阳性对照组相比,高剂量DFP+Al组睾丸组织中SOD活力较高,MDA含量较低,差异均有统计学意义(P<0.05或P<0.01)。高、中、低剂量DFP+Al组睾丸组织中SOD活力和MDA含量与阴性对照组相比,差异无统计学意义(P>0.05)。高、中、低剂量DFP+Al组和阴性对照组大鼠睾丸组织中的铝含量均低于阳性对照组,差异有统计学意义(P<0.01)。各组大鼠睾丸组织中Fe、Zn、Ca、Mg、Cu元素含量间比较,差异均无统计学意义(P>0.05)。结论DFP对染铝大鼠受损睾丸组织抗氧化系统有保护作用,且在排铝的同时,对睾丸组织中必需元素铁、锌、钙、镁、铜的代谢平衡无影响。
Objective To investigate tile effect of 1,2-dimethyl-3-hydroxy-4-pyridone （DFP） on anti-oxidant system,and levels of aluminum （Al）,iron （Fe）,zinc （Zn）,copper （Cu）,calcium （Ca） and magnesium （Mg） in the testicles of rats exposed to aluminum. Methods Thirty-five male Wistar rats were randomly divided into five groups： the negative control group,low DFP, moderate and high doses groups and positive group. After 8 weeks of treatment with aluminum chloride at the dose of 172.5 mg/（kg·d ）, the positive group was given 1 ml normal saline, while DFP groups were given DFP solution at the doses of 13.82,27.44 and 54.88 mg/（ kg·d ）. The drugs were given by garage, once a day, 5 days a week. Twenty-four hours after the last gavage, the coefficients of testicles were calculated. The activity of superoxide dismutase （SOD）,the contents of malondialdehyde （MDA）, Al, Fe,Zn, Ca, Mg and Cu in the testicle were determined. Results No significant difference was seen among all groups in the weight of bodies or testicles and coefficients of testicles. With the DFP dose increased,the activity of SOD in the testicle showed an increasing trend, while MDA content presented a decreasing trend. The activity of SOD was higher and MDA content was lower in aluminum＋high dose DFP group than positive group （P〈0.05 or P〈0.01）.Al level in the testicles of positive group was significantly higher than that in the other groups （P〈0.01）. No significant defference was observed among all groups in the contents of Fe,Zn,Ca,Mg and Cu. Conclusion DFP can protect the antioxidant system in the testicle exposed to aluminum, in the process of expelling aluminum with DFP, no obvious adverse effect on the essential elements in the testicle was observed.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2009年第4期290-292,共3页
Journal of Environment and Health
基金
山东省自然科学基金资助项目(Y2006C35)
山东省济南市青年科技明星计划项目(200205)
