摘要
目的探讨枯草芽胞杆菌的芽胞在肉鸡肠道中的生活状态和分布。方法以20日龄AA肉鸡为研究对象,饲喂枯草芽胞菌剂,分别测定鸡粪中芽胞数量和鸡肠道不同部位的活菌数量。结果饲喂3 h后,鸡粪中开始检测到芽胞的存在,24 h达到最高值,直至饲喂120 h后,肠道内的芽胞基本排除。排出芽胞总量为饲喂芽胞总数的3.0倍左右,同时研究还表明:芽胞在实验肉鸡的十二指肠2内开始萌发,并进行了繁殖,在小肠的后端,即小肠3和小肠4,活菌数量达到高峰。结论部分芽胞进入小肠后即可开始萌发,并进行生长繁殖,而且在肠道内有短暂滞留。
Objective To learn the fate and dissemination of B. subtilis spores in gastrointestinal tract of broiler. Method Bacillus subtilis spores were fed to 20-day-old AA broilers. The number of Bacillus subtilis spores in faeces and in different parts of gastrointestinal tract of broiler were measured. Result B. subtilis spores were detectable by 3 h after inoculation;the majority of counts occurred within the first 24 h. No B. subtilis were detected in the 120 h samples. The number of B. subtilis spores CFU recovered from the faeces was 200% more than the original inoculum. The results also showed that B. subtilis spores started to germinate and reproduce in the duodenum 2. The live B. subtilis reached to peak in the smaU intestine 3 and the small intestine 4. Conclusion A proportion of the spores had germinated and undergone one or more rounds of growth and replication, and spores may colonize the small intestine, albeit briefly.
出处
《中国微生态学杂志》
CAS
CSCD
2009年第4期334-336,共3页
Chinese Journal of Microecology
关键词
枯草杆菌
芽胞
肉鸡
肠道
Bacillus subtilis
Spore
Broiler
Gastrointestinal tract
