摘要
目的:探讨内皮抑素对人肝癌细胞株HepG-2细胞生长和血管内皮生长因子(VEGF)表达的影响及其可能的机制。方法:采用MTT法检测不同浓度的内皮抑素作用不同时间后对HepG-2细胞生长的抑制作用;电镜观察加药前后HepG-2细胞超微结构的变化;免疫组织化学法检测内皮抑素作用前后HepG-2细胞中存活素表达的改变;RT-PCR法检测加药前后HepG-2细胞中VEGF基因表达的变化。结果:内皮抑素能抑制HepG-2细胞的生长增殖,呈剂量-时间依赖性,并诱导细胞凋亡;内皮抑素作用后HepG-2细胞中存活素表达明显减少,VEGF表达明显减少。结论:内皮抑素通过下调存活素的表达,诱导HepG-2细胞的凋亡、抑制其增殖,并能显著降低HepG-2细胞中VEGF的表达。
Objective： To evaluate the inhibitory effect and possible mechanisms of endostatin on HepG-2 cells and the expression of VEGF, and to provide experimental evidence for clinical treatment of hepatoma with endostatin. Methods： MTT assay was used to observe the inhibitory effects of endostatin on HepG-2 cells at various concentrations and different time points. Ultrastructural changes of HepG-2 were examined under an electron microscopy. The expression of survivin protein was analyzed by immunocytochemistry before and after endostatin treatment. VEGF mRNA content was determined by RTPCR test. Results： The growth of HepG-2 cells was significantly inhibited by endostatin in a dose-time-dependent fashion and significant apoptosis was presented. Immunohistochemical results showed that the expression of survivin protein decre assed obviously after being treated by endostation. The expression of VEGF in HepG-2 cells was inhibited by endostatin. Conclusion ： Endostatin can inhibit the proliferation and induce apoptosis of HepG-2 cells by decreasing expression of survivin protein, and endostatin can inhibit the expression of VEGF.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2009年第2期159-161,共3页
Chinese Journal of Anatomy
关键词
内皮抑素
肝癌细胞
凋亡
存活素
血管内皮生长因子
endostatins
HepG 2 cell line
apoptosis
survivin
vascular endothelial growth factor
