摘要
农杆菌介导法在果树遗传改良中有广阔的应用前景,农杆菌介导法的受体系统有原生质体、愈伤组织、直接分化芽和胚状体受体系统等;影响农杆菌转化频率的因素有侵染力,抗生素,基因型等;常用检测方法有GUS活性检测,PCR技术,杂交检测,免疫学检测。从农杆菌介导法在果树中的各种转化受体系统、影响果树基因转化的主要因素和转化植株的检测等方面进行综述,为不同果树建立高效的基因转化体系提供参考。
Agrobacterium-mediated method has broad application prospects in the genetic improvement of fruit direct bud differentiation receptor system and embryoid Body systems. The factors of affecting the frequency of Agrobacterium-mediated transformation include infection, antibiotics; genotype. Commonly methods of testing include GUS activity detection, PCR technology, hybridization, and immunological detection. This article is reviewed on the Agrobacterium-mediated in a variety receptor system of fruit trees, transforming ways, main factors affecting transfermation of fruit genes and the detections and so on, and provide references for different fruit trees to establish highly efficient gene transfer systems.
出处
《中国农学通报》
CSCD
北大核心
2009年第9期179-183,共5页
Chinese Agricultural Science Bulletin
基金
湖南省重点课题"转基因rol在梨树上的表达及效应研究"(04TT1007)
关键词
受体系统
农杆菌
侵染力
检测
receptor system, Agrobacterium, infective ability, detection
