摘要
为了获得高产植酸酶菌株,利用PCR技术,从黑曲霉WP1中克隆出2种植酸酶基因phyA和phyB,分别命名为phyA1、phyB1。phyA1长1 346 bp,成熟肽序列不含内含子,共编码448个氨基酸;phyB1基因长1 560 bp,基因序列含有3段内含子,共编码460个氨基酸。phyA1和phyB1序列同源性很低,只有21.12%。二者都含有1个植酸酶基因保守序列RHGXRXP;但phyA1含2个HD保守序列,phyB1只含有1个。将黑曲霉WP1植酸酶phyA1和phyB1的基因序列在国际基因库中注册,注册号分别为:DQ650711;DQ836360。
In order to get a high phytase activity strain,the phyAl and phyB1 encoding phytase of Aspergill WP1 were amplified by the polymerase chain reaction（PCR）with primers designed according to the sequences us niger in Gene Bank. The amplified fragments were cloned and sequenced. The results showed that the coding region were 1 346 bp and 1 560 bp in size, there was no intron in phyA1 gene, encodes a peptide of 448 amino acids;but phyBl includes 3 introns, and encodes a peptide of 460 amino acid residues. The area of highest homology RHGXRXP was found in the two sequences. There were two HD in phyAl and only one in phyB1 . The phyA1 and phyBl of A. niger WP1 strain in this paper their sequences have been accessed by Gene Bank（Accession：DQ650711 ;DQ836360）.
出处
《华北农学报》
CSCD
北大核心
2009年第2期22-26,共5页
Acta Agriculturae Boreali-Sinica
基金
河北省"十一五"科技攻关项目资助(06220106D)
