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利福平、异烟肼合用与单用时的肝毒性比较 被引量:7

Comparison of Hepatotoxicity Induced by Isoniazid,Rifampicin or Isoniazid and Combined with Rifampicin
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摘要 目的:探讨异烟肼(INH)与利福平(RFP)合用致肝毒性增加的部分作用机制。方法:采用在体心脏灌流法获取小鼠原代肝细胞,将其常规培养72h后,含INH(87.5μmol/L)、RFP(48.6μmol/L)、INH+RFP(87.5μmol/L+48.6μmol/L)培养液培养48h后,常规HE染色,光镜下观察肝细胞生长情况并计数。结果:INH与RFP合用后与INH单用时比较肝细胞计数减少(P<0.05,P<0.01)。结论:RFP和INH合用后使INH肝毒性代谢物浓度增加,可能是其肝毒性增加的原因之一。 Objective: To investigate the mechanism of the effect of Isoniazid (INH)and Rifampicin (RFP). Methods: The mouse hepatocytes were obtained through the Cardio-perfusion in mouse. Mouse hepatocytes were isolated by two-step in situ collagenase perfusion methods,72 hours after culture of mouse hepatocytes were cultivated, the culture liquid with INH (87.5 μmol/L), RFP(48.6 μmol/L) , INH + RFP(87.5 μmol/L + 48.6 μmol/L)were cultivated for 48 hours,routine HE dyeing and growth of hepatocytes were observed and counted under electromicroscope. Results: Hepatotoxicity of INH was lower when Combined with RFP than with INF alone ( P 〈 0.05, P 〈 0. 01 ). Conclusion:RFP can increase the concentration of Acetylhydrazin (AHZ)and Hydrazine, which may increase the hepatotoxicity of INH.
出处 《华北国防医药》 2009年第2期27-28,F0003,共3页 Medical Journal of Beijing Military Region
关键词 异烟肼 利福平 原代培养肝细胞 肝毒性 Isoniazid Rifampicin Hepatocyte primary culture Hepatotoxicity
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