摘要
目的研究尿路感染病原菌中产超广谱β-内酰胺酶菌的耐药状况，为临床合理用药提供参考。方法收集本院2008年1月～2008年12月尿液标本分离出的病原菌326株，用Kirby-Bauer纸片扩散法进行抗菌药物的敏感性实验，ESBLs纸片法筛选和确证试验。操作按NCCLS-2006年标准进行。结果326株病原菌中分离出革兰阴性杆菌223株，其中产ESBLs的有99株，占44．40％，分别为大肠埃希菌73株，肺炎克雷伯菌26株。产ESBLs大肠埃希菌对20种抗菌药的敏感性排在前3位的是美罗培南（100％）、亚胺培南-西司他丁（100％）、阿米卡星（84．9％）。产ESBLs肺炎克雷伯菌对20种抗菌药物的敏感性排在前3位的是芙罗培南（100％）、亚胺培南-西司他丁（100％）、哌拉西林-他唑巴坦（73．1％）。结论本院尿路感染病原菌中分离出的产超广谱β-内酰胺酶菌多重耐药率高，临床应参考药敏结果，合理选用抗菌药物。
Objective To investigate the antibiotic resistance of extended-spectrum β-lactamase producing strains among pathogens in urinary tract infection, and to provide some reference for reasonable use of antibiotics. Methods A total of 326 samples of pathogens was collected and separated from the urine specimens from January 2008 to December 2008 in our hospital, the sensitivity experiment to antimicrobial with Kriby-Bauer test, ESBLs confirmatory test were carried-out according to the standard of NCCLS-2006. Results All the 326 pathogenic strains were identified, 223 of which were Gram-negative bacteria, and 99 ESBLs-producing strains were detected in gram-negative bacteria. The detection rate was 44.40 %, 73 of which were Escherichia coli, and 26 were Klebsiella pneumonia. The top 3 susceptible antimicrobial drugs for the 73 strains of Escherichia coli were meropenem （ 100%）, imipenem （ 100% ）, and amikacin （ 84.9% ） ; the top 3 susceptible antimicrobial drugs for the 26 strains of Klebsiella pneumoniae were meropenem （ 100 % ）, imipenem （ 100% ）, and piperacillin/tazobactam （ 73.1% ）. Conclusion The extended-spectrum β-lactamase producing strains separated from pathogens in urinary tract infection in our hospital has a high multiple drug resistance. Doctors should choose appropriate antimicrobials according to antimicrobial susceptibility tests.
出处
《中南药学》
CAS
2009年第4期311-313,共3页
Central South Pharmacy
关键词
尿路感染
产超广谱Β-内酰胺酶菌
耐药性
urinary tract infection
extended-spectrum β-lactamase producing strains
drug resistance
