摘要
目的探讨不同分化程度的胃腺癌细胞对5-氟尿嘧啶和奥沙利铂的敏感性。方法以不同浓度的5-氟尿嘧啶和奥沙利铂分别处理低分化和中分化胃腺癌细胞,采用四甲基偶氮唑盐比色法(M TT法)检测药物对胃腺癌细胞增殖的影响。结果5-氟尿嘧啶和奥沙利铂分别对2种胃腺癌细胞的增殖均具有显著的抑制作用,且随药物浓度增加而增强,中分化的胃腺癌细胞对2种化疗药物的敏感性均明显高于低分化的胃腺癌细胞。结论不同浓度的5-氟尿嘧啶和奥沙利铂对胃腺癌细胞的抑制作用不同,不同分化程度的胃腺癌细胞对5-氟尿嘧啶和奥沙利铂的敏感性也有不同。
Objective To investigate the sensitivity of fluorouracil or oxaliplatin in gastric adenocarcinoma cell by MTT assay. Methods Poorly differentiated and moderately differentiated gastric adenocarcinoma cells were incubated with fluorouracil or oxaliplatin of various concentration. The cytotoxicity was examined by MTT test. Results Fluorouracil or oxaliplatin in different concentration had significant killing effects on two kinds of gastric adenocarcinoma ceils in vitro. Moderately differentiated gastric adenocarcinoma ceils had a higher sensitivity to ehemotherapeutics than poorly differentiated cells with the increase in concentration. Conclusion The inhibiting effects of paclitaxel or oxaliplatin in various concentrations were different on gastric adenocarcinoma cells and the sensitivity of fluorouracil or oxaliplatin were different between the two degrees of differentiated cells.
出处
《中国药业》
CAS
2009年第9期18-19,共2页
China Pharmaceuticals
