摘要
目的观察肝纤维化不同证型与基质金属蛋白酶(MMPs)及其抑制因子的关系。方法清洁级雄性SD大鼠140只,依随机数字表法分为4组:正常对照组20只、单纯肝纤维化组30只、肝纤维化肝郁脾虚证组、肝纤维化气虚血瘀证组各45只。各模型组先用四氯化碳注射法复制肝硬化疾病模型,然后肝郁脾虚证组附加夹尾和大黄灌胃法、气虚血瘀证组附加游泳疲劳法,并以相应药物干预。采用免疫组化法测定肝组织基质金属蛋白酶2(MMP-2)、基质金属蛋白酶9(MMP-9)和金属蛋白酶抑制剂2(TIMP-2)水平。结果各模型组肝组织中MMP-2、MMP-9和TIMP-2均较正常组明显升高(P<0.01或P<0.05),以气虚血瘀组最为明显。结论基质金属蛋白酶及其抑制因子与肝纤维化密切相关,在不同中医证型中表达有差异,可作为肝纤维化中医辨证分型及疗效评价的指标之一。
Objective To observe the different expressions of matrix metalloproteinases （MMPs） and inhibitory factor in different TCM syndromes of liver fibrosis. Methods The 140 male SD rats were randomized into 4 groups： 20 in normal control group, 30 in simple liver fibrosis group, 45 in liver fibrosis liver stagnation and spleen deficiency group, and 45 in liver fibrosis qi deficiency and blood stasis group. All rats of model groups were treated by CCI4 to establish the liver fibrosis models first, and the rats of liver stagnation and spleen deficiency group were treated by stimulating tails with forceps and gastric infusion of rhubarb decoction, and the rats of qi deficiency and blood stasis group were treated by the method of swimming-fatigue. Then each group was intervened by the relevant medicine. After treatment, the Matrix Metalloproteinases-2 （MMP-2）, Matrix Metalloproteinases-9 （MMP-9） and Tissue Inhibitors of Metalloprotienas-2 （TIMP-2） of liver were examined. Results The MMP-2, MMP-9 and TIMP-2 of model groups were significantly higher than those of the control group （P〈0. 01 or P〈0.05）, and the increase of those of qi deficiency and blood stasis group were most obvious. Conclusion The MMPs and inhibitory factor closely relate with liver fibrosis, their expression in different TCM syndromes is different, serving as one of indexes for differentiation of TCM syndromes and evaluating therapeutic effect.
出处
《中医杂志》
CSCD
北大核心
2009年第4期343-346,共4页
Journal of Traditional Chinese Medicine
基金
浙江省医药卫生科学研究基金项目计划(2005A077)
