摘要
目的探讨锁阳多糖、水提取物咀嚼片延缓衰老的效应及其作用机制。方法提取并制备锁阳多糖、水提取物咀嚼片;Wistar大鼠随机分为空白对照组、衰老模型组、维生素E对照组、锁阳多糖组、水提物组,以D-半乳糖建立衰老大鼠模型,药物干预后,MTT法检测各组大鼠脾淋巴细胞对ConA刺激的增殖能力,检测各组大鼠脾脏指数,观察脾组织的病理形态学变化;并检测各组大鼠血清中超氧化物歧化酶(SOD)活性、丙二醛(MDA)及一氧化氮(NO)的含量。结果与正常组比较,衰老模型组大鼠脾脏指数、脾淋巴细胞对ConA刺激的增殖能力明显下降,脾脏发生明显病理损伤,血清中SOD活性明显下降,MDA及NO含量显著升高(P<0.05),锁阳提取物咀嚼片能使上述指标明显逆转(P<0.05)。结论锁阳提取物咀嚼片对D-半乳糖所致衰老模型大鼠脾脏病理变化、脾淋巴细胞免疫功能,自由基代谢相关指标等具有明显改善作用。
Objective To explore the anti-aging effect and mechanism of the Cynomorium water extract chewable tablets and Cynomofium polysaecharide chewable tablets. Methods The Cynomorium polysaccharide and Cynomorium water extract were extracted and prepared chewable tablets. Wistar rats were randomly divided into normal control, aging model, VitE, Cynomorium polysaecharide chewable tablets and Cynomorium water extract chewable tablets groups. D-galactose （D-gal） was injected to establish the aging model. After drug intervention, the proliferation activity of rat spleen lymphocytes stimulated by ConA was detected by MTF assay and the spleen index was measured. The pathological morphological changes of spleen were observed. The activity of superoxide dismutase （SOD） , the content of malondialdehyde （MDA） and nitric oxide （ NO ） in serum of rats were detected. Results Compared with the normal control group, the spleen index, the proliferation activity of rat spleen lymphocytes stimulated by ConA of aging model rats were significantly lower, the spleen had obvious pathological injury, and the activity of SOD was significantly lower, the content of MDA and NO increased significantly （ all P 〈 0. 05 ）. Cynomorium chewable tablets made above abnormal indexes significant improve （P 〈 0. 05）. Conclusions The extraction of Cynomorium chewable tablets has significant improvement on the immunopathologic change and free radical metabolism of aging rats model induced by D-gal.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2009年第8期927-929,共3页
Chinese Journal of Gerontology
基金
甘肃省教育厅科研基金资助项目(034B-02)
关键词
衰老
锁阳
脾脏
免疫
自由基
Aging
Cynomorium Songaricum Rupr.
Spleen
Immune
Free radical
