摘要
目的研究纯的裸露磁性纳米Fe3O4颗粒对肝癌细胞SMMC-7721和人肺腺癌细胞SPC-A-1 2种细胞活性的影响。方法2种细胞与浓度为0~0.25 mg/ml的纯磁性纳米Fe3O4颗粒培育不同时间后,利用噻唑蓝法（MTT）对细胞的活性进行检测;同时通过显微镜观察和原子吸收法分别对细胞吞噬磁性纳米颗粒的效果及其吞噬量进行分析。结果当浓度不高于100μg/ml时,未经修饰的纯磁性纳米Fe3O4颗粒不会对SMMC-7721和SPC-A-1细胞的生长产生明显抑制作用,同时SPC-A-1细胞对纳米颗粒的吞噬量也较SMMC-7721的低。结论裸露磁性纳米Fe3O4颗粒在较大浓度范围内对SMMC-7721和SPC-A-1细胞的生长和代谢无明显的影响,这为磁性纳米Fe3O4颗粒在许多生物医学领域中的应用提供了实验参考和依据。
Objective To investigate the effect of the pure magnetic Fe3O4 nanopartilces on the human hepatoma cells SMMC- 7721 and human lung adenocarcinoma cells SPC-A-1. Methods After SMMC-7721 cells and SPC-A-1 cells incubated with fresh medium containing pure magnetic Fe3O4 nanopartilces in different concentrations （range 0 -0.25mg/ml）, cell viability was examined with MTT assays, and the intracellular uptake amount into cells and iron content within the cells were analyzed by microscopy and atomic absorption spectrophotometry（AAS） respectively. Results The pure magnetic Fe3O4 nanopartilces did not cause apparent effect on the growth of the SMMC-7721 and SPC-A-1 cells at the concentrations not higher than 100μg/mL, and the intracellular uptake amount into the SPC-A-1 cells was relatively lower than the SMMC-7721. Conclusion The pure magnetic Fe3O4 nanopartilces are biocompatible to the cells when they are incubated in the definite concentration, and no evidence show that there are adverse effects on the growth and metabolism of the cells. It would provide an intensive experimental foundation for the application of magnetic nanoparticles.
出处
《毒理学杂志》
CAS
CSCD
北大核心
2009年第2期89-91,92,共4页
Journal of Toxicology
基金
国家自然科学基金(c03031801)
上海市优秀学科带头人计划(06XD14039)
关键词
磁性纳米颗粒
细胞
活性
Magnetic nanopartiele
Cell
Effect
