摘要
本研究旨在筛选高产ＤＨＡ的异养型微藻种．以含有５ｇ／Ｌ葡萄糖的Ｐｏｒｐｈｙｒｉｄｉｕｍ培养基为初始培养基，对四株异养双鞭藻———Ｃｒｙｐｔｈｅｃｏｄｉｎｉｕｍｃｏｈｎｉ进行摇床培养筛选．其中ＣｒｙｐｔｈｅｃｏｄｉｎｉｕｍｃｏｈｎｉＡＴＣＣ３０５５６的比生长速率（０．０６７／ｈ）、生物量（２．０４６ｇ／Ｌ）以及ＤＨＡ产量（１５９ｍｇ／Ｌ）均为最高．筛选结果表明，该藻种具有进一步研究的意义及进行工业化生产的可能．
WT5”BZ〗Four strains of the heterotrophic dinoflagellate Crypthecodinium cohnii were screened for their docosahexaenoic acid (DHA) production potential using shake flask cultures on Porphyridium medium with glucose (5 g/L). Of the four strains tested, Crypthecodinium cohnii ATCC 30556 had the highest specific growth rate (0.067/h), the highest biomass concentration (2.046 g/L) and the highest DHA yield (159 mg/L). The screening results showed that C. cohnii ATCC 30556 was the most promising strain for further study and for industrial production of DHA.
出处
《华南理工大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
1998年第4期17-22,共6页
Journal of South China University of Technology(Natural Science Edition)
基金
香港研究基金
香港政府工业署资助课题
广东省自然科学基金
关键词
二十二碳六烯酸
微藻
异养
docosahexaenoic acid
Crypthecodinium cohnii
microalgae
heterotrophic
