摘要
[目的]改进松辽黑猪脏器总RNA的高效快速提取方法,为检测脏器组织RNA提供基础。[方法]采用液氮研磨,利用Trizol使细胞膜破裂,并通过氯仿、异丙醇、无水乙醇处理,使RNA与杂质分离,得到高质量的RNA。[结果]得到的松辽黑猪脏器总RNA浓度和纯度都比较高,并且未被降解。[结论]该方法提取的RNA质量没有DNA污染、纯度高、没有降解、完整性好,稳定可靠,并且用时少。
[Objective] The aim was to improve an effective and rapid method for extracting total RNA of Songliao black pig, and provide basis for testing
organs tissue RNA. [Method] Using liquid nitrogen grinding method, cell membrane was ruptured by using Trizol , through some treatments with chloroform, isopropanol and absolute alcohol, RNA was purified and high quality RNA was obtained. [Result] The concentration and purity were high of obtained total RNA of Songliao black pig, and were not degraded. [Conclusion] The quality and purity of RNA are high and not contaminated by DNA by using this method, which has better integrity and stability, and could save time.
出处
《安徽农业科学》
CAS
北大核心
2009年第12期5384-5385,共2页
Journal of Anhui Agricultural Sciences
基金
国家科技支撑计划项目(2007BAD55B03)
国家高技术研究发展计划项目(2008AA10Z136)
关键词
松辽黑猪
脏器
总RNA
提取
Songliao black pig
Organ
Total RNA
Extraction
