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利用16S～23S rDNA间隔序列对葡萄酒中的酒酒球菌的鉴定 被引量：1

Identification of Oenococcus oeni in Grape Wine by Use of 16S～23S rDNA Ribosomal-Spacer-Region
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摘要 对从宁夏银川地区自然发酵的葡萄酒中分离得到30株菌株,并对菌株进行分子鉴定、生理生化鉴定和酿酒适应性的筛选。从中优选出4株菌株,对筛选出的4株菌株分别进行了PCR扩增,得到它们的16S～23SrDNAITS片段,并测定所得到片段的DNA序列。将测定结果与GenBankDNA数据库已知的菌种对比,确定4株菌株均为酒酒球菌。 30 strains were collected from the grape wine made in Yinchuan, Ningxia by natural fermentation. Then 4 strains were screened among them by molecular identification, biochemical identification, and wine-making adaptability screening. Then the 16S-23S rDNA ITS fragments of the 4 strains were obtained after they were amplified by PCR. And the DNA sequence of the obtained fragments were determined and the determinatoin results were compared with the known strains in GenBank DNA database. The 4 strains finally were identified as Oenococcus oeni.
作者 周利国 刘树文 李西柱 王坐京
机构地区 西北农林科技大学葡萄酒学院 陕西省葡萄与葡萄酒工程技术研究中心
出处 《酿酒科技》 2009年第5期39-41,共3页 Liquor-Making Science & Technology
关键词 微生物 菌株鉴定 16S^23S rDNA间隔序列 PCR 葡萄酒 酒酒球菌 microbe strain identification 16S-23S rDNA Ribosomal-Spacer-Region PCR grape wine Ooenococcus oeni
分类号 Q93-3 [生物学—微生物学] Q78 [生物学—分子生物学]
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参考文献7

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二级参考文献26

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共引文献10

同被引文献30

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  • 3刘延琳,李华.酒酒球菌的快速特异性PCR鉴定[J].微生物学杂志,2006,26(6):26-29. 被引量：3
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酿酒科技
2009年 第5期

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