摘要
采用北京市农林科学院畜牧兽医研究所制备的ND-IB-EDS三联灭活疫苗,分别以0.1,0.2,0.3,0.5mL/只的剂量免疫3周龄SPF鸡,免后3周测定ND及EDSHI抗体,然后用NDV强毒北京株攻毒;另外,首免用H120活苗免疫SPF鸡,二免分别用0.1～0.5mL/只三联苗,并测定2次免疫后IBHI抗体。试验结果表明,0.1mL/只的免疫剂量就可产生较好的免疫效果,因此,确定该疫苗的最小免疫剂量为0.1mL/只。
3-week-old SPF chickens were immunized with triplex oil emulsion vaccine ND-IB-EDS with different doses of 0.1, 0.2, 0.3 and 0.5 mL prepared by institute of Animal Husbandry and Veterinary Medicine of Beijing Academy of Agriculture and Forestry. ND and EDS HI antibodies in chickens were detected after immunization for 3 weeks and the chickens were challenged with virulent NDV Beijing strains; In addition, SPF chickens were immunized with H 120 live vaccine at first immunization and that were immunized with triplex oil emulsion vaccine at 0.1 - 0.5 mL per chicken resp. at second immunization, and then the IB HI antibodies in chickens were measured after two immunities. The results showed that immune dose of 0.1 mL could produce better immune effect and therefore the minimum immune dose of the ND-IB-EDS vaccine was 0.1 mL per chicken.
出处
《畜牧与饲料科学》
2009年第3期128-129,共2页
Animal Husbandry and Feed Science
关键词
鸡新城疫
传染性支气管炎
减蛋综合征
三联灭活疫苗
最小免疫剂量
newcastle disease
infectious bronchitis
egg drop syndrome
triplex oil emulsion vaccine
minimum immune dose
