期刊文献+

过氧化氢处理时间对异种骨移植替代物的影响 被引量:3

INFLUENCE OF IMMERSED TIME BY HYDROGEN DIOXIDE ON XENOGENEIC BONE SUBSTITUTE
原文传递
导出
摘要 目的探讨H2O2处理时间对牛松质骨粒生物学特性的影响。方法健康24月龄秦川牛10头,雌雄不限,体重150~170kg。取牛肱骨头松质骨,切割成5mm×5mm×5mm大小骨粒,分别以8.8mol/LH2O2浸泡0、12、24、36、48、60、72h后,行灰化组分测定、扫描电镜观察、X射线能谱分析及显微CT观察,研究牛松质骨粒的组分、结构、骨质及骨量变化。结果随H2O2处理时间延长,骨粒有机物含量逐渐减少,0~24h和60~72h有机物含量差异有统计学意义(P<0.05);24~60h无明显变化(P>0.05)。骨粒中钙、磷元素含量逐渐减少,60h后几乎检测不到(P<0.05)。骨密度、骨矿物质含量呈下降趋势,且60h后下降明显(P<0.05);骨小梁逐渐变细,骨小梁分离度增加。结论H2O2能有效去除异种骨粒中有机物抗原成分,但处理时间超过60h会严重破坏骨粒组分和结构,故合理掌握处理时间对消除异种骨移植替代物抗原性并维持其生物学特性具有重要意义。 Objective To study the in uence of the immersed time by hydrogen dioxide on the characteristics of bovine cancellous bone granules in various periods.Methods Ten 24-month-old Qinchuan bovine, male or female, weighing 150-170 g, were selected.Cancellous bone granules from metaphysic of bovine long bone were cut into cubes of 5 mm × 5 mm × 5 mm and immersed by 8.8 mol/L hydrogen dioxide for 0, 12, 24, 36, 48, 60 and 72 hours separately.Determination of ash, scanning electron microscope, X-ray energy spectrum and micro CT were used to investigate the changes of composition, structure and quality of bone.Results With the immersed time increasing, the contents of organics in the bone cancellous were reduced gradually, and obviously decreased during the periods of 0 to 24 hours and 60 to 72 hours(P 〈 0.05).The contents of calcium and phosphorus decreased gradually, they could not be detected almost after 60 days(P 〈 0.05).Bone mineral density and bone mineral content were decreased obviously after 60 hours(P 〈 0.05).The bone trabecula became slimmer and trabecular spacing became larger.Conclusion Hydrogen dioxide can be used to remove the antigen in xenogeneic bone;however as the time increasing(more than 60 hours) the composition and structure will be damaged.Thus it is important to control the immersed time for maintaining the biological characteristics of xenogeneic bone substitute as well as eliminating antigen by hydrogen dioxide.
出处 《中国修复重建外科杂志》 CAS CSCD 北大核心 2009年第5期538-541,共4页 Chinese Journal of Reparative and Reconstructive Surgery
关键词 过氧化氢 异种骨 灰分 扫描电镜 显微CT X射线能谱 Hydrogen dioxide Xenogeneic bone Ash determination Scanning electron microscope Micro CT X-ray energy spectrum
  • 相关文献

参考文献19

  • 1Montjovent MO, Mathieu L, Schmoekel H, et al. Repair of critical size defects in the rat cranium using ceramic-reinforced PLA scaffolds obtained by supercritical gas foaming. J Biomed Mater Res A, 2007, 83(1): 41-51.
  • 2奚廷斐.生物医用材料现状和发展趋势[J].中国医疗器械信息,2006,12(5):1-4. 被引量:23
  • 3Vanaclocha V, Bazan A, Saiz-Sapena N, et al. Use of frozen cranial vault bone allografts in the repair of extensive cranial bone defects. Acta Neurochir, 1997, 139(7): 653-660.
  • 4Ellis E 3rd, Sinn DP. Use of homologous bone in maxillofacial surgery. J Oral Maxillofac Surg, 1993, 51(11): 1181-1193.
  • 5Johnson GS, Mucalo MR, Lorier MA. The processing and characterization of animal-derived bone to yield materials with biomedical applications. Part h Modifiable porous implants from bovine condyle cancellous bone and characterization of bone materials as a function of processing. J Mater Sci Mater Med, 2000, 11(7): 427-441.
  • 6Guizzardi S, Martini D, Bacchelli B, et al. Effects of heat deproteinate bone and polynucleotides on bone regeneration: an experimental study on rat. Micron, 2007, 38(7): 722-728.
  • 7Nyman JS, Reyes M, Wang X. Effect of ultrastructural changes on the toughness of bone. Micron, 2005, 36(7-8): 566-582.
  • 8Anderson IA, Mucalo MR, Johnson GS, et al. The processing and characterization of animal derived bone to yield materials with biomedical applications. Part III: material and mechanical properties of fresh and processed bovine cancellous bone. J Mater Sci Mater Med, 2000, 11(11): 743-749.
  • 9Giraud Guille MM, Mosser G, Helary C, et al. Bone matrix like assem- blies of collagen: From liquid crystals to gels and biomimetic materials. Micron, 2005, 36(7-8): 602-608.
  • 10Fritsch A, Hellmich C. ‘Universal' microstructural patterns in cortical and trabecular, extracellular and extravascular bone materials: Micromechanics-based prediction of anisotropic elasticity. J Theor Biol, 2007, 244(4): 597-620.

二级参考文献9

共引文献73

同被引文献36

引证文献3

二级引证文献11

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部