摘要
目的:探讨肝素对深Ⅱ度烧伤小鼠内源性碱性成纤维生长因子(bFGF)和血小板源生长因子(PDGF)表达的影响。方法:制作小鼠深Ⅱ度烫伤模型,肝素组用3000U/mL肝素外敷伤口2min,每日2次,观察创面愈合状况,并在给药后第3天分别从肝素组(烫伤后用肝素处理)、烫伤组(烫伤后自然愈合)及对照组(未进行烫伤处理)取5只小鼠处死,提取烫伤及其周围皮肤组织的蛋白质,用Western-blotting检测bFGF和PDGF的表达状况。结果:肝素组bFGF和PDGF表达均高于烫伤组;而烫伤组的两种生长因子的表达均高于对照组。结论:烫伤后bFGF和PDGF的表达量增高,且肝素能促进深Ⅱ度烧伤内源性生长因子bFGF、PDGF的表达。
Objective： To determine the effects of heparin on the expression of growth factors of bFGF and PDGF at the deep second-degree bums. Methods： The mice with deep second-degree burned mice were divided into three groups randomly, Heparin treated group （3000U/mL heparin after burning）, burned group （the wound without treatment） and control group （no bums）. Expression of growth factors of bFGF and PDGF was measured by western-blotting. Results： Expression of PDGF and bFGF was higher in Heparin treated group than that in burned group, both of which were higher than those in control group. Conclusions： Heparin stimulated the expression of internal growth factors （bFGF, PDGF） at the deep second-degree bums.
出处
《现代生物医学进展》
CAS
2009年第9期1660-1662,共3页
Progress in Modern Biomedicine
