摘要
将3株新城疫病毒（NDV）内蒙分离株纯化培养后，提取病毒RNA，经RT-PER扩增F基因，并测定出F基因的全部核苷酸序列为1662bp，编码有554个氨基酸残基。与国内外发表的部分NDV的F基因核苷酸序列进行比较，结果表明这3株分离株核苷酸同源性分别为97．5％，97．9％和98．0％，与标准毒株F48E9的同源性分别为85．8％，86．4％和86．8％，与长春、河北、山东、广东、广西、浙江、韩国等地的NDV的同源性在96．3％～98．8％之间。并且这3株分离株F蛋白的裂解位点氨基酸组成为^112R-R-Q-K-R-F^117，具有典型的强毒株裂解位点的特点。
The fusion （F） protein genes of three newcastle disease virus （NDV） isolates from Inner Mongolia were amplified by RT-PCR and sequenced. The sequencing analysis showed that the F gene encoding 554 amino acids was 1 662 bp in length. The homologous compari- son of the three isolates showed 97.5%, 97.9% and 98.0% of identities for each other; 85.8% , 86. 4% and 86. 8% with F48E9 strain, re- spectively; 96. 3% -98.8% with the isolates from Changchun, Hebei, Shandong, Guangdong, Guangxi, Zhejiang and South Korea. The cleavage sites （ 112 - 117 ） of F protein from the three isolates were ,^112R-R-Q-K-R-F^117 , which is characteristic of the velogenic strain.
出处
《畜牧与兽医》
北大核心
2009年第5期25-28,共4页
Animal Husbandry & Veterinary Medicine
