摘要
目的:测定新资源短梗五加及糙叶藤五加、红毛五加、刺五加、白毛五加、韩五加、细柱五加、白簕五加等8种五加属植物根皮中紫丁香苷的含量。方法:采用RP-HPLC测定紫丁香苷的含量。色谱柱为ODSC18柱,流动相为20%甲醇溶液,流速为1.0ml/min,检测波长为265nm,柱温35℃。采用正交试验法考察乙醇浓度、提取时间、溶剂用量对提取效果的影响。结果:紫丁香苷在0.0148～0.074mg/ml(r=0.9999)范围内,进样量与峰面积具有良好的线性关系,回归方程:Y=15234560X-7881.767,R2=0.9999,平均回收率为98.24%。8种五加属植物中,糙叶藤五加紫丁香苷含量最高,短梗五加次之。最佳提取工艺为用水做提取剂,料液比1:15、煮沸提取3.5h。结论:本法简便,结果可靠,可用于新资源短梗五加及其同属植物的质量控制。
The objective of this study was to determine the contents of syringin in 8 species ofAcanthopanax Miq. such as A. sessiliflorus （Rupr. et Maxim.） Seem, A. leucorrhizus （Oliv.） Harms var.fulvescens Harms et Rehd., Acanthopanax giraldii Harms, A. senticosus （Rupr. et Maxin） Harms, A. divaricatus var. albeofructus Yook, A. koreanum Nakai, A. gracilistylus W.W. Smith, and A.trifoliatus （L.） Merr by RP-HPLC. The column, mobile phase, flow rate, detection RP-HPLC were ODS C18 as An orthogonal experimental design was employed to optimize the extraction conditions of syringin. The method displayed good linearity within the concentration range of 0.0148 to 0.074 mg/ml （r = 0.9999） and a regression equation of Y = 15234560 X - 7 881.767 （R^2 = 0.9999） was obtained. The average spike recovery was 98.24%. Acanthopanax leucorrhizus var.fulvescens Harms et Rehd. Had the highest content of syringin among the 8 species ofAcanthopanax Miq., followed by A.sessiliflorus （Rupr. et Maxim.） Seem. The optimum extraction process of syringin was adding material to 15 times volume water and then boiling the water for 3.5 h. Our results suggested the simplicity and high reliability of this method to analyze syringin.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第10期140-143,共4页
Food Science
基金
教育部留学回国人员科研启动基金项目(教外司留[2005]55号)
关键词
短梗五加
五加属植物
RP—HPLC
紫丁香苷
A. sessiliflorus （Rupr. et Maxim.） Seem： Acanhopanax Miq.
RP-HPLC
syringin
