摘要
以乳清粉为原料,采用胃蛋白酶酶解进行预处理,膜过滤提取β-乳球蛋白。研究酶解过程及膜分离过程的主要因素,确定最佳的酶解条件和提取条件。所得产品用SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)和紫外-可见分光光度法进行定性和定量检测。结果表明,最佳酶解条件为:温度50℃、pH值为2.5、酶用量为0.2%、固液比为1∶5、酶解时间为1h,膜分离条件为:pH值为7、膜操作压力0.25MPa。此条件下所得产品纯度为93.6%,提取率为65.3%。
β-1actoglobulin（β-Lg） was successfully extracted with a unique method. The method comprised a peptic treatment of solution of whey powder and membrane filtration under gentle condition for isolation of nativeβ-Lg. The main factors was studied and the qualitative and quantitative analysis of different extracted β- Lg was carried out by SDS-PAGE and ultraviolet spectrophotometry. Results presented demonstrate that the optimal pretreatment conditions were 30 ℃, 60 min, pH2.5, enzyme concentration 0.2%, solid-liquid ratio 1：5, and membrane filtration conditions were pH 7, 0.25 MPa. At this condition the β-Lg purity is 93.6%, the yield is 65.3%.
出处
《食品科技》
CAS
北大核心
2009年第5期245-248,251,共5页
Food Science and Technology
